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The WRKY domain is a 60 amino acid region that is defined by the conserved amino acid sequence WRKYGQK at its N-terminal end, together with a novel zinc-finger-like motif. The WRKY domain is found in one or two copies in a superfamily of plant transcription factors involved in the regulation of various physiological programs that are unique to plants, including pathogen defense, senescence, trichome development and the biosynthesis of secondary metabolites. The WRKY domain binds specifically to the DNA sequence motif (T)(T)TGAC[CT], which is known as the W box. The invariant TGAC core of the W box is essential for function and WRKY binding [1].

Some proteins known to contain a WRKY domain are listed below:

• Arabidopsis thaliana Zinc-dependent Activator Protein-1 (ZAP1).
• Arabidopsis thaliana AtWRKY44/TTG2, a protein involved in trichome development and anthocyanin pigmentation.
• Wild oat ABF1-2, two proteins involved in the gibberelic acid-induced expression of the α-Amy2 gene.

The profile we developed covers the entire WRKY domain.

May 2002 / First entry.

PROSITE method (with tools and information) covered by this documentation:

WRKY, PS50811; WRKY domain profile  (MATRIX)

Sequences known to belong to this class detected by the profile: ALL Other sequence(s) detected in Swiss-Prot: NONE

• Domain architecture view of Swiss-Prot proteins matching PS50811 • Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS50811 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS50811 • Scan Swiss-Prot/TrEMBL entries against PS50811 • view ligand binding statistics

Matching PDB structures: 1WJ2 2AYD 2LEX [ALL]

1	Authors	Eulgem T., Rushton P.J., Robatzek S., Somssich I.E.
	Title	The WRKY superfamily of plant transcription factors.
	Source	Trends Plant Sci. 5:199-206(2000).
	PubMed ID	10785665

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