{PDOC50876} {PS50876; ZF_INTEGRASE} {BEGIN} ************************************** * Zinc finger integrase-type profile * ************************************** The retroviral integrase is the enzyme responsible for the insertion of a DNA copy of the viral genome into host DNA, an essential step in the replication cycle of viruses [1]. Integrases comprise three functional and structural domains: the central core domain, which contains the catalytic site, an N-terminal zinc finger and a C-terminal DNA binding domain [2]. The zinc finger has the signature of zinc-binding residues H-x(3)-H-x(23)-C-x(2)-C. The function of this domain is unclear; however, it is required for integration activity and enhances tetramerization in the context of the full-length integrase [3]. The structure of this domain has been solved [3], it comprises four helices (see ). The fold is very similar to that of a number of HTH DNA binding motifs, with helices 2 and 3 corresponding to the HTH motif . The third helice is used for dimerization, whereas in the HTH motif it binds DNA. The profile we developed covers the whole domain. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: April 2004 / First entry. [ 1] Frankel A.D., Young J.A. "HIV-1: fifteen proteins and an RNA." Annu. Rev. Biochem. 67:1-25(1998). PubMed=9759480; DOI=10.1146/annurev.biochem.67.1.1 [ 2] Esposito D., Craigie R. "HIV integrase structure and function." Adv. Virus. Res. 52:319-333(1999). PubMed=10384240 [ 3] Zheng R., Jenkins T.M., Craigie R. "Zinc folds the N-terminal domain of HIV-1 integrase, promotes multimerization, and enhances catalytic activity." Proc. Natl. Acad. Sci. U.S.A. 93:13659-13664(1996). PubMed=8942990 [ 4] Cai M., Zheng R., Caffrey M., Craigie R., Clore G.M., Gronenborn A.M. "Solution structure of the N-terminal zinc binding domain of HIV-1 integrase." Nat. Struct. Biol. 4:567-577(1997). PubMed=9228950 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}