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Yeast FCP1 is an essential protein serine phosphatase (EC 3.1.3.16) that dephosphorylates the C-terminal domain (CTD) of RNA polymerase II. FCP1 orthologs are present in all known eukaryote proteomes. The N-terminal domain of FCP1 corresponds to the catalytic unit of the phosphatase and has been refered to as the FCP1 homology domain. The FCP1 homology domain, which is a ~180-residue module, is also found in many other proteins of unknown function. It contains a DxDx(T/V) motif preceded by four hydrophobic residues characteristic of a large family of metal-dependent phosphohydrolases and phosphotransferases. The first aspartate residue is likely to participate in catalysis, whereas the second could have a role in substrate recognition [1,2,3,4].

The profile we developed covers the entire FCP1 homology domain.

March 2004 / First entry.

PROSITE method (with tools and information) covered by this documentation:

FCP1, PS50969; FCP1 homology domain profile  (MATRIX)

Sequences known to belong to this class detected by the profile: ALL Other sequence(s) detected in Swiss-Prot: NONE

• Domain architecture view of Swiss-Prot proteins matching PS50969 • Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS50969 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS50969 • Scan Swiss-Prot/TrEMBL entries against PS50969 • view ligand binding statistics

Matching PDB structures: 1T9Z 1TA0 2GHQ 2GHT ... [ALL]

1	Authors	Archambault J., Chambers R.S., Kobor M.S., Ho Y., Cartier M., Bolotin D., Andrews B., Kane C.M., Greenblatt J.
	Title	An essential component of a C-terminal domain phosphatase that interacts with transcription factor IIF in Saccharomyces cerevisiae.
	Source	Proc. Natl. Acad. Sci. U.S.A. 94:14300-14305(1997).
	PubMed ID	9405607

2	Authors	Kobor M.S., Archambault J., Lester W., Holstege F.C.P., Gileadi O., Jansma D.B., Jennings E.G., Kouyoumdjian F., Davidson A.R., Young R.A., Greenblatt J.
	Title	An unusual eukaryotic protein phosphatase required for transcription by RNA polymerase II and CTD dephosphorylation in S. cerevisiae.
	Source	Mol. Cell 4:55-62(1999).
	PubMed ID	10445027

3	Authors	Hausmann S., Shuman S.
	Title	Defining the active site of Schizosaccharomyces pombe C-terminal domain phosphatase Fcp1.
	Source	J. Biol. Chem. 278:13627-13632(2003).
	PubMed ID	12556522
	DOI	10.1074/jbc.M213191200

4	Authors	Cho H., Kim T.-K., Mancebo H., Lane W.S., Flores O., Reinberg D.
	Title	A protein phosphatase functions to recycle RNA polymerase II.
	Source	Genes Dev. 13:1540-1552(1999).
	PubMed ID	10385623

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