{PDOC51765}
{PS51765; ZF_RAG1}
{BEGIN}
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* Zinc finger RAG1-type profile *
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The development of B and T cells depends on the rearrangement of variable (V),
diversity (D),  and  joining (J) gene segments to produce mature Ig and T cell
receptor coding   regions.   This   rearrangement   process,  known  as  V(D)J
recombination is  initiated  by  the  complex,  multi-domain proteins RAG1 and
RAG2. The  RAG  proteins  catalyze  DNA  cleavage  in  the  first phase of the
reaction using  a  recombination  signal sequence (RSS) that flanks V, D and J
segments [1,2,3].

RAG1 contains a zinc-binding dimerization domain immediately N-terminal to the
catalytic core  region,  which  in turn contains a putative DNA-binding domain
(see <PDOC51487>)  at  its  N  terminus. The dimerization domain consists of a
zinc C3HC4 RING finger (see <PDOC00449>) and a C2H2 zinc RAG1-type finger.

The C2H2  RAG1-type  zinc  finger  contains  the hallmarks of a classical zinc
finger structure  with  a  two  stranded  beta-sheet  and  an alpha-helix (see
<PDB:1RMD>) [1].

The profile we developed covers the entire RAG1-type zinc finger.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: July 2015 / First entry.

[ 1] Bellon S.F., Rodgers K.K., Schatz D.G., Coleman J.E., Steitz T.A.
     "Crystal structure of the RAG1 dimerization domain reveals multiple
     zinc-binding motifs including a novel zinc binuclear cluster."
     Nat. Struct. Biol. 4:586-591(1997).
     PubMed=9228952
[ 2] Jones J.M., Gellert M.
     "Autoubiquitylation of the V(D)J recombinase protein RAG1."
     Proc. Natl. Acad. Sci. U.S.A. 100:15446-15451(2003).
     PubMed=14671314; DOI=10.1073/pnas.2637012100
[ 3] Mao M.-G., Lei J.-L., Alex P.-M., Hong W.-S., Wang K.-J.
     "Characterization of RAG1 and IgM (mu chain) marking development of
     the immune system in red-spotted grouper (Epinephelus akaara)."
     Fish Shellfish Immunol. 33:725-735(2012).
     PubMed=22796426; DOI=10.1016/j.fsi.2012.06.011

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