{PDOC51917}
{PS51917; PRU}
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* Pru (pleckstrin-like receptor for the Ub) domain profile *
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In eukaryotes,  selective  protein  degradation  is performed primarily by the
ubiquitin–proteasome pathway,  in  which  substrate  proteins  are  marked  by
covalently attached   ubiquitin   chains   that  mediate  recognition  by  the
proteasome. Rpn13  (also  called  ADRM1)  is  a subunit of the proteasome that
serves as  a  receptor  for  both ubiquitin and Uch37, one of the proteasome's
three deubiquitinating  enzymes.  Rpn13  binds  ubiquitin  though  a conserved
amino-terminal region  termed  the  pleckstrin-like  receptor for the Ub (Pru)
domain (also  known as the ubiquitin-binding domain, or UBD), which binds K48-
linked diubiquitin. Its carboxy-terminal DEUBAD domain (see <PDOC51916>) binds
deubiquinating enzyme  Uch37/UCHL5  and  enhances  its  isopeptidase  activity
[1,2,3].

The Pru   domain   forms  two  contiguous,  antiparallel  beta-sheets  with  a
configuration similar  to  the  pleckstrin-homology structural domain: a four-
stranded twisted   antiparallel   beta-sheet   (beta   1,  4-6)  packs  almost
orthogonally against   a   second   triple  stranded  beta-sheet  (beta  7-8).
Juxtaposed to  the  three-stranded  sheet are two beta-strands (beta 2-3). The
Pru domain forms a hydrophobic core containing conserved hydrophobic residues,
which are  located  within beta-sheets. One end of the beta-sandwich is capped
by a long C-terminal amphipatic alpha-helix (see <PDB:2R2Y>) [1,2,3].

The profile we developed covers the entire Pru domain.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: March 2020 / First entry.

[ 1] Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y.,
     Hofmann K., Walters K.J., Finley D., Dikic I.
     "Proteasome subunit Rpn13 is a novel ubiquitin receptor."
     Nature 453:481-488(2008).
     PubMed=18497817; DOI=10.1038/nature06926
[ 2] Schreiner P., Chen X., Husnjak K., Randles L., Zhang N., Elsasser S.,
     Finley D., Dikic I., Walters K.J., Groll M.
     "Ubiquitin docking at the proteasome through a novel
     pleckstrin-homology domain interaction."
     Nature 453:548-552(2008).
     PubMed=18497827; DOI=10.1038/nature06924
[ 3] Chen X., Lee B.-H., Finley D., Walters K.J.
     "Structure of proteasome ubiquitin receptor hRpn13 and its activation
     by the scaffolding protein hRpn2."
     Mol. Cell. 38:404-415(2010).
     PubMed=20471946; DOI=10.1016/j.molcel.2010.04.019

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