|PROSITE documentation PDOC50804 [for PROSITE entry PS50804]|
This domain of around 80 amino acid residues has been called LeR because it is a leucine rich region , or SCAN box from the first letter of the names of the four proteins initially found to contain it (SRE-ZBP, CTfin-51, AW-1, Number 18 cDNA) . The SCAN box is essentially found in the N-terminal part of transcription factors containing a zinc finger of the C2H2 type (see <PDOC00028>). These proteins can either activate or repress transcription, although isolated recombinant SCAN boxes do not modulate significantly the transcription [1,2]. In addition to these zinc finger transcription factors, an isolated SCAN box without adjacent zinc finger motifs has been identified in some proteins [3,4].
The SCAN box is enriched in hydrophobic and negatively charged residues with a L-X(6)-L motif at its core. This core is flanked by A, E, L, M, H and C residues that are frequently found in α-helices . Predictions of the secondary structure of the domain suggest the presence of at least three α-helices that are separated from one another by short looped regions bounded by proline residues . It has been shown to be a selective oligomerization domain that mediates homotypic and heterotypic interactions between SCAN box containing proteins [4,5].
The following zinc-finger proteins have been shown to contain a SCAN box:
And a number of proteins devoid of C2H2-type zinc-finger domain:
The profile we developed spans the complete SCAN box.Last update:
December 2001 / First entry.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Pengue G., Calabro V., Bartoli P.C., Pagliuca A., Lania L.|
|Title||Repression of transcriptional activity at a distance by the evolutionarily conserved KRAB domain present in a subfamily of zinc finger proteins.|
|Source||Nucleic Acids Res. 22:2908-2914(1994).|
|2||Authors||Williams A.J., Khachigian L.M., Shows T., Collins T.|
|Title||Isolation and characterization of a novel zinc-finger protein with transcription repressor activity.|
|Source||J. Biol. Chem. 270:22143-22152(1995).|
|3||Authors||Castillo G., Brun R.P., Rosenfield J.K., Hauser S., Park C.W., Troy A.E., Wright M.E., Spiegelman B.M.|
|Title||An adipogenic cofactor bound by the differentiation domain of PPARgamma.|
|Source||EMBO J. 18:3676-3687(1999).|
|4||Authors||Schumacher C., Wang H., Honer C., Ding W., Koehn J., Lawrence Q., Coulis C.M., Wang L.L., Ballinger D., Bowen B.R., Wagner S.|
|Title||The SCAN domain mediates selective oligomerization.|
|Source||J. Biol. Chem. 275:17173-17179(2000).|
|5||Authors||Williams A.J., Blacklow S.C., Collins T.|
|Title||The zinc finger-associated SCAN box is a conserved oligomerization domain.|
|Source||Mol. Cell. Biol. 19:8526-8535(1999).|