{PDOC00071} {PS00074; GLFV_DEHYDROGENASE} {BEGIN} **************************************************** * Glu / Leu / Phe / Val dehydrogenases active site * **************************************************** - Glutamate dehydrogenases (EC 1.4.1.2, EC 1.4.1.3, and EC 1.4.1.4) (GluDH) are enzymes that catalyze the NAD- or NADP-dependent reversible deamination of glutamate into alpha-ketoglutarate [1,2]. GluDH isozymes are generally involved with either ammonia assimilation or glutamate catabolism. - Leucine dehydrogenase (EC 1.4.1.9) (LeuDH) is a NAD-dependent enzyme that catalyzes the reversible deamination of leucine and several other aliphatic amino acids to their keto analogues [3]. - Phenylalanine dehydrogenase (EC 1.4.1.20) (PheDH) is a NAD-dependent enzyme that catalyzes the reversible deamidation of L-phenylalanine into phenyl- pyruvate [4]. - Valine dehydrogenase (EC 1.4.1.8) (ValDH) is a NADP-dependent enzyme that catalyzes the reversible deamidation of L-valine into 3-methyl-2- oxobutanoate [5]. These dehydrogenases are structurally and functionally related. A conserved lysine residue located in a glycine-rich region has been implicated in the catalytic mechanism. The conservation of the region around this residue allows the derivation of a signature pattern for such type of enzymes. -Consensus pattern: [LIV]-x(2)-G-G-[SAG]-K-x-[GV]-x(3)-[DNST]-[PL] [K is the active site residue] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Note: All known sequences from this family have Pro in the last position of the pattern with the exception of yeast GluDH which as Leu. -Last update: November 1997 / Pattern and text revised. [ 1] Britton K.L., Baker P.J., Rice D.W., Stillman T.J. "Structural relationship between the hexameric and tetrameric family of glutamate dehydrogenases." Eur. J. Biochem. 209:851-859(1992). PubMed=1358610 [ 2] Benachenhou-Lahfa N., Forterre P., Labedan B. J. Mol. Evol. 36:335-346(1993). [ 3] Nagata S., Tanizawa K., Esaki N., Sakamoto Y., Ohshima T., Tanaka H., Soda K. "Gene cloning and sequence determination of leucine dehydrogenase from Bacillus stearothermophilus and structural comparison with other NAD(P)+-dependent dehydrogenases." Biochemistry 27:9056-9062(1988). PubMed=3069133 [ 4] Takada H., Yoshimura T., Ohshima T., Esaki N., Soda K. "Thermostable phenylalanine dehydrogenase of Thermoactinomyces intermedius: cloning, expression, and sequencing of its gene." J. Biochem. 109:371-376(1991). PubMed=1880121 [ 5] Tang L., Hutchinson C.R. "Sequence, transcriptional, and functional analyses of the valine (branched-chain amino acid) dehydrogenase gene of Streptomyces coelicolor." J. Bacteriol. 175:4176-4185(1993). PubMed=8320231 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}