{PDOC00131}
{PS00321; RECA_1}
{PS50162; RECA_2}
{PS50163; RECA_3}
{BEGIN}
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* recA family signature and profiles *
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The bacterial   recA   protein   [1,2,3]   is   essential   for  homologous
recombination and   recombinational  repair  of  DNA  damage.  RecA  has  many
activities: it  filaments,  it  binds  to  single- and double-stranded DNA, it
binds and hydrolyzes ATP,  it is also a recombinase and, finally, it interacts
with lexA causing its activation and leading to its autocatalytic cleavage.

RecA is  a protein of about 350 amino-acid residues. Its sequence is very well
conserved [3,4,5]    among  eubacterial  species.  It  is also found in the
chloroplast of plants [6].

The recA protein is closely related to:

 - Eukaryotic  RAD51  protein. Promotes homologous pairing and strand exchange
   on chromatin.
 - Eukaryotic DMC1 protein. Participates in meiotic recombination.
 - Prokaryotic  radA  protein.  Involved  in  DNA  repair  and  in  homologous
   recombination.
 - Bacteriophage  uvsX  gene product. Important  in genetic recombination, DNA
   repair, and replication.

As a  signature  pattern  specific  for  the  bacterial and chloroplastic recA
protein, we  selected  the best conserved region, a nonapeptide located in the
middle of the sequence and which is part of the monomer-monomer interface in a
recA filament.

We also  developed   two profiles. The first one covers the ATP binding domain
in the N-terminal part of  the  recA  protein. The  second  one span the whole
monomer-monomer  interface.  These  two profiles  also  pick up  the recA-like
proteins.

-Consensus pattern: A-L-[KR]-[IF]-[FY]-[STA]-[STAD]-[LIVMQ]-R
-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Sequences known to belong to this class detected by the profile: ALL.
 recA-like eukaryotic proteins.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Expert(s) to contact by email:
           Roca A.I.;
roca@macc.wisc.edu

           Eisen J.A.;
jeisen@leland.stanford.edu

-Last update: December 2012 / Profile revised.

[ 1] Smith K.C., Wang T.-C.
     "recA-dependent DNA repair processes."
     BioEssays 10:12-16(1989).
     PubMed=2653307
[ 2] Lloyd A.T., Sharp P.M.
     "Evolution of the recA gene and the molecular phylogeny of bacteria."
     J. Mol. Evol. 37:399-407(1993).
     PubMed=8308907
[ 3] Roca A.I., Cox M.M.
     Prog. Nucleic Acids Res. Mol. Biol. 56:129-223(1997).
[ 4] Karlin S., Weinstock G.M., Brendel V.
     "Bacterial classifications derived from recA protein sequence
     comparisons."
     J. Bacteriol. 177:6881-6893(1995).
     PubMed=7592482
[ 5] Eisen J.A.
     "The RecA protein as a model molecule for molecular systematic studies
     of bacteria: comparison of trees of RecAs and 16S rRNAs from the same
     species."
     J. Mol. Evol. 41:1105-1123(1995).
     PubMed=8587109
[ 6] Cerutti H.D., Osman M., Grandoni P., Jagendorf A.T.
     "A homolog of Escherichia coli RecA protein in plastids of higher
     plants."
     Proc. Natl. Acad. Sci. U.S.A. 89:8068-8072(1992).
     PubMed=1518831

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