{PDOC00173} {PS00195; GLUTAREDOXIN_1} {PS51354; GLUTAREDOXIN_2} {BEGIN} ********************************************************* * Glutaredoxin active site signature and domain profile * ********************************************************* Glutaredoxin [1,2,3], also known as thioltransferase, is a small protein of approximately one hundred amino-acid residues. It functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. Like thioredoxin, which functions in a similar way, glutaredoxin possesses an active center disulfide bond. It exists in either a reduced or an oxidized form where the two cysteine residues are linked in an intramolecular disulfide bond. Glutaredoxin has been sequenced in a variety of species. On the basis of extensive sequence similarity, it has been proposed [4] that vaccinia protein O2L is most probably a glutaredoxin. Finally, it must be noted that phage T4 thioredoxin seems also to be evolutionary related. The pattern is directed against the 2 cysteines of the redox active bonds. We also developed a profile that covers the whole glutaredoxin domain. -Consensus pattern: [LIVMD]-[FYSA]-x(4)-C-[PV]-[FYWH]-C-x(2)-[TAV]-x(2,3)- [LIV] [The 2 C's form the redox-active bond] -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Note: In position 5 of the pattern, all glutaredoxin sequences have Pro while T4 thioredoxin has Val. -Last update: December 2007 / Text revised and profile added. [ 1] Gleason F.K., Holmgren A. "Thioredoxin and related proteins in procaryotes." FEMS Microbiol. Rev. 4:271-297(1988). PubMed=3152490 [ 2] Holmgren A. "Thioredoxin and glutaredoxin: small multi-functional redox proteins with active-site disulphide bonds." Biochem. Soc. Trans. 16:95-96(1988). PubMed=3286320 [ 3] Holmgren A. "Thioredoxin and glutaredoxin systems." J. Biol. Chem. 264:13963-13966(1989). PubMed=2668278 [ 4] Johnson G.P., Goebel S.J., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E. "Vaccinia virus encodes a protein with similarity to glutaredoxins." Virology 181:378-381(1991). PubMed=1994586 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}