{PDOC00201} {PS00229; TAU_MAP_1} {PS51491; TAU_MAP_2} {BEGIN} ********************************************************************* * Tau and MAP proteins tubulin-binding repeat signature and profile * ********************************************************************* Microtubules consist of tubulins as well as a group of additional proteins collectively known as the Microtubule Associated Proteins (MAP). MAP's have been classified into two classes: high molecular weight MAP's and Tau protein. These proteins promote microtubule assembly and stabilize microtubules. The C-terminal region of a subset of these proteins contains three or four tandem repeats of a conserved domain of about thirty amino acid residues which is implicated in tubulin-binding and which seems to have a stiffening effect on microtubules. The proteins currently known to contain such repeats are: - Tau [1], from neurones. - MAP2 [2], a neuronal member of the high molecular weight MAP's. - MAP4 [3], a non-neuronal member of the high molecular weight MAP's. MAP4 is is also expressed in some neurons. The pattern we developed to detect this repeated region spans the last thirteen residues of the repeated region. We also developed a profile which covers the entire repeat. -Consensus pattern: G-S-x(2)-N-x(2)-H-x-[PA]-[AG]-G(2) -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Note: The first repeat of MAP2 is not picked up by the pattern because it has Tyr instead of His in position 8, and Lys instead of Gly in position 11. -Expert(s) to contact by email: Matus A.; matus@fmi.ch -Last update: May 2010 / Profile added. [ 1] Kosik K.S., Orecchio L.D., Bakalis S., Neve R.L. "Developmentally regulated expression of specific tau sequences." Neuron 2:1389-1397(1989). PubMed=2560640 [ 2] Matus A. "Stiff microtubules and neuronal morphology." Trends Neurosci. 17:19-22(1994). PubMed=7511844 [ 3] Chapin S.J., Bulinski J.C. "Non-neuronal 210 x 10(3) Mr microtubule-associated protein (MAP4) contains a domain homologous to the microtubule-binding domains of neuronal MAP2 and tau." J. Cell Sci. 98:27-36(1991). PubMed=1905296 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}