{PDOC00378} {PS00463; ZN2_CY6_FUNGAL_1} {PS50048; ZN2_CY6_FUNGAL_2} {BEGIN} ***************************************************************** * Zn(2)-C6 fungal-type DNA-binding domain signature and profile * ***************************************************************** A number of fungal transcriptional activator proteins contain a cysteine-rich region that binds DNA in a zinc-dependent fashion. It has been shown [1,2] that this region forms a binuclear zinc cluster where six conserved cysteines bind two zinc cations. Some proteins known to contain such a domain are listed below [3]. Gene name(s) Biological source Scope of activatory action --------------- --------------------- --------------------------------- aflR Aspergillus Aflatoxin biosynthesis amdR or intA Emericella nidulans Amides catabolism ARGR2 or ARG81 Yeast Arginine metabolism CAT8 or MSP8 Yeast Gluconeogenic enzymes CBF3B or CEP3 Yeast Chromosome segregation CHA4 Yeast L-serine dehydratase CYP1 or HAP1 Yeast Iso-1 and iso-2 cytochrome c CZF1 Candida albicans Unknown GAL4 Yeast Galactose metabolism LAC9 Kluyveromyces lactis Galactose metabolism LEU3 Yeast Leucine biosynthesis LYS14 Yeast Lysine biosynthesis MAL1R or MAL13 Yeast Maltose metabolism MAL3R or MAL33 Yeast Maltose metabolism MAL6R or MAL63 Yeast Maltose metabolism nirA Emericella nidulans Nitrate assimilation nit-4 Neurospora crassa Nitrate assimilation PDR1 or CYH3 Yeast Permeability PDR3 Yeast Permeability ? PIP2 Yeast Peroxisome proliferation PPR1 Yeast Pyrimidine metabolism PUT3 Yeast Proline metabolism qa-1f Neurospora crassa Quinate metabolism qutA Emericella nidulans Quinate metabolism SEF1 Yeast & K.lactis ? SIP4 Yeast Unknown STB5 Yeast Unknown SUC1 Candida albicans Sucrose utilization TEA1 Yeast Ty1 enhancer binding thi1 or ntf1 Fission yeast Thiamine repressible genes THI2 Yeast Thiamine biosynthesis uaY Emericella nidulans Purine utilization UGA3 Yeast GABA catabolism UGA35 or DAL81 Yeast GABA, urea, arginine, allantoin and catabolism UME6 Yeast Many genes such as arginase, sporulation genes, etc. YAL051w Yeast ? (Chromosome I) YBR033w Yeast ? (Chromosome II) YBR150c Yeast ? (Chromosome II) YBR239c Yeast ? (Chromosome II) YCR106w Yeast ? (Chromosome III) YDR213c Yeast ? (Chromosome IV) YDR303c Yeast ? (Chromosome IV) YDR421w Yeast ? (Chromosome IV) YDR520c Yeast ? (Chromosome IV) YER184c Yeast ? (Chromosome V) YFL052w Yeast ? (Chromosome VI) YHR056c Yeast ? (Chromosome VIII) YIL130w Yeast ? (Chromosome IX) YJL103c Yeast ? (Chromosome X) YJL206c Yeast ? (Chromosome X) YKL038w Yeast ? (Chromosome XI) YKL222c Yeast ? (Chromosome XI) YKR064w Yeast ? (Chromosome XI) YLR278c Yeast ? (Chromosome XII) YML076c Yeast ? (Chromosome XIII) YMR019w Yeast ? (Chromosome XIII) YNR063w Yeast ? (Chromosome XIV) YPL133c Yeast ? (Chromosome XVI) The spacing of the cysteines in this type of domain is C-x(2)-C-x(6)-C-x(5 to 12)-C-x(2)-C-x(6 to 8)-C. There are also a number of other conserved residues in the loop regions between the cysteines which can be used to help define a specific pattern for such a domain. This domains is generally found in the N-terminal section of these proteins with the exception of UME6 where it is found in the C-terminal section. -Consensus pattern: [GASTPV]-C-x(2)-C-[RKHSTACW]-x(2)-[RKHQ]-x(2)-C-x(5,12)-C- x(2)-C-x(6,8)-C [The 6 C's are zinc ligands] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: human ultra high-sulfur keratin. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: March 2005 / Text revised. [ 1] Pan T., Coleman J.E. "GAL4 transcription factor is not a 'zinc finger' but forms a Zn(II)2Cys6 binuclear cluster." Proc. Natl. Acad. Sci. U.S.A. 87:2077-2081(1990). PubMed=2107541 [ 2] Marmorstein R., Carey M., Ptashne M., Harrison S.C. "DNA recognition by GAL4: structure of a protein-DNA complex." Nature 356:408-414(1992). PubMed=1557122; DOI=10.1038/356408a0 [ 3] Schjerling P., Holmberg S. "Comparative amino acid sequence analysis of the C6 zinc cluster family of transcriptional regulators." Nucleic Acids Res. 24:4599-4607(1996). PubMed=8967907 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}