{PDOC00411} {PS00446; RNA_POL_D_30KD} {BEGIN} ****************************************************** * RNA polymerases D / 30 to 40 Kd subunits signature * ****************************************************** In eukaryotes, there are three different forms of DNA-dependent RNA polymerases (EC 2.7.7.6) transcribing different sets of genes. Each class of RNA polymerase is an assemblage of ten to twelve different polypeptides. In archaebacteria, there is generally a single form of RNA polymerase which also consist of an oligomeric assemblage of 10 to 13 polypeptides. It has been shown [1,2,3] that small subunits of about 30 to 40 Kd found in all three types of eukaryotic polymerases are highly conserved. Subunits known to belong to this family are: - Budding yeast RPC5 subunit (or RPC40) from RNA polymerases I and III. - Mammalian RPA40 from RNA polymerase I. - Budding yeast RPB3 subunit from RNA polymerase II. - Fission yeast rpb3 subunit from RNA polymerase II. - Mammalian RPB3 (or RPB33) (gene POLR2C) from RNA polymerase II. - Conjugation stage-specific protein cnjC from Tetrahymena thermophila, which may be a stage-specific RNA polymerase subunit. - Archebacterial RNA polymerase subunit D (gene rpoD). As a signature pattern for these subunits, we selected a conserved region in their N-terminal part. -Consensus pattern: N-[SGAT]-[LIVMF]-R-R-x(9)-[SAR]-x(3)-[VA]-x(4)-N-x-[STA]- x(3)-[DN]-E-x-[LIV]-[GSA]-x-R-[LI]-[GAS]-[LIVM](2)-[PV] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: December 2004 / Pattern and text revised. [ 1] Kolodziej P., Young R.A. "RNA polymerase II subunit RPB3 is an essential component of the mRNA transcription apparatus." Mol. Cell. Biol. 9:5387-5394(1989). PubMed=2685562 [ 2] Pati U.K., Weissman S.M. "The amino acid sequence of the human RNA polymerase II 33-kDa subunit hRPB 33 is highly conserved among eukaryotes." J. Biol. Chem. 265:8400-8403(1990). PubMed=2187864 [ 3] Azuma Y., Yamagishi M., Ishihama A. "Subunits of the Schizosaccharomyces pombe RNA polymerase II: enzyme purification and structure of the subunit 3 gene." Nucleic Acids Res. 21:3749-3754(1993). PubMed=8367291 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}