{PDOC00447}
{PS00516; ALKYLBASE_DNA_GLYCOS}
{BEGIN}
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* Alkylbase DNA glycosidases alkA family signature *
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Alkylbase DNA glycosidases [1] are  DNA repair  enzymes  that   hydrolyzes the
deoxyribose N-glycosidic bond to excise various alkylated bases from a damaged
DNA polymer.

In Escherichia coli there  are  two alkylbase DNA glycosidases: one (gene tag)
which is constitutively expressed and  which is specific for the removal of 3-
methyladenine (EC 3.2.2.20),  and  one (gene alkA)  which  is  induced  during
adaptation to alkylation and which can remove a variety of alkylation products
(EC 3.2.2.21). Tag and alkA do not share any region of sequence similarity.

In yeast there is  an alkylbase DNA glycosidase (gene MAG1) [2,3],  which  can
remove 3-methyladenine or 7-methyladenine and which is structurally related to
alkA.

MAG and alkA are both proteins of about 300 amino acid residues.  While the C-
and N-terminal ends appear to be unrelated, there is a central region of about
130 residues which is well conserved.  As a signature pattern we have selected
part of this region.

-Consensus pattern: G-[IV]-[GK]-x-W-[ST]-[AVI]-x-[LIVMFY](2)-x-[LIVM]-x(8)-
                    [MF]-x(2)-[ED]-D
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: December 2004 / Pattern and text revised.

[ 1] Lindahl T., Sedgwick B., Sekiguchi M., Nakabeppu Y.
     "Regulation and expression of the adaptive response to alkylating
     agents."
     Annu. Rev. Biochem. 57:133-157(1988).
     PubMed=3052269; DOI=10.1146/annurev.bi.57.070188.001025
[ 2] Berdal K.G., Bjoras M., Bjelland S., Seeberg E.C.
     "Cloning and expression in Escherichia coli of a gene for an alkylbase
     DNA glycosylase from Saccharomyces cerevisiae; a homologue to the
     bacterial alkA gene."
     EMBO J. 9:4563-4568(1990).
     PubMed=2265619
[ 3] Chen J., Derfler B., Samson L.
     "Saccharomyces cerevisiae 3-methyladenine DNA glycosylase has homology
     to the AlkA glycosylase of E. coli and is induced in response to DNA
     alkylation damage."
     EMBO J. 9:4569-4575(1990).
     PubMed=2265620

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