{PDOC00504} {PS00583; PFKB_KINASES_1} {PS00584; PFKB_KINASES_2} {BEGIN} ************************************************** * pfkB family of carbohydrate kinases signatures * ************************************************** It has been shown [1,2,3] that the following carbohydrate and purine kinases are evolutionary related and can be grouped into a single family, which is known [1] as the 'pfkB family': - Fructokinase (EC 2.7.1.4) (gene scrK). - 6-phosphofructokinase isozyme 2 (EC 2.7.1.11) (phosphofructokinase-2) (gene pfkB). pfkB is a minor phosphofructokinase isozyme in Escherichia coli and is not evolutionary related to the major isozyme (gene pfkA). Plants 6-phosphofructokinase also belong to this family. - Ribokinase (EC 2.7.1.15) (gene rbsK). - Adenosine kinase (EC 2.7.1.20) (gene ADK). - 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45) (gene: kdgK). - 1-phosphofructokinase (EC 2.7.1.56) (fructose 1-phosphate kinase) (gene fruK). - Inosine-guanosine kinase (EC 2.7.1.73) (gene gsk). - Tagatose-6-phosphate kinase (EC 2.7.1.144) (phosphotagatokinase) (gene lacC). - Escherichia coli hypothetical protein yeiC. - Escherichia coli hypothetical protein yeiI. - Escherichia coli hypothetical protein yhfQ. - Escherichia coli hypothetical protein yihV. - Bacillus subtilis hypothetical protein yxdC. - Yeast hypothetical protein YJR105w. All the above kinases are proteins of from 280 to 430 amino acid residues that share a few region of sequence similarity. We have selected two of these regions as signature patterns. The first pattern is based on a region rich in glycine which is located in the N-terminal section of these enzymes; while the second pattern is based on a conserved region in the C-terminal section. -Consensus pattern: [AG]-G-x(0,1)-[GAP]-x-N-{AGLS}-[STA]-x(2)-{A}-x-{G}- {GNKA}-[GS]-x(9)-G -Sequences known to belong to this class detected by the pattern: ALL, except for ADK, gsk, kdgK, yeast ribokinase and YJR105w, B.subtilis yxdC and E.coli yihV. -Other sequence(s) detected in Swiss-Prot: 33. -Consensus pattern: [DNSK]-[PSTV]-x-[SAG](2)-[GD]-D-x(3)-[SAGV]-[AG]- [LIVMFYA]-[LIVMSTAP] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: 5. -Note: Some bacterial fructokinases belong to the ROK family . -Last update: April 2006 / Pattern revised. [ 1] Wu L.-F., Reizer A., Reizer J., Cai B., Tomich J.M., Saier M.H. Jr. "Nucleotide sequence of the Rhodobacter capsulatus fruK gene, which encodes fructose-1-phosphate kinase: evidence for a kinase superfamily including both phosphofructokinases of Escherichia coli." J. Bacteriol. 173:3117-3127(1991). PubMed=1850730 [ 2] Orchard L.M.D., Kornberg H.L. "Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus." Proc. R. Soc. Lond., B, Biol. Sci. 242:87-90(1990). PubMed=1981619 [ 3] Blatch G.L., Scholle R.R., Woods D.R. "Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region." Gene 95:17-23(1990). PubMed=2174811 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}