{PDOC00583} {PS00696; ETF_ALPHA} {BEGIN} ********************************************************** * Electron transfer flavoprotein alpha-subunit signature * ********************************************************** The electron transfer flavoprotein (ETF) [1,2] serves as a specific electron acceptor for various mitochondrial dehydrogenases. ETF transfers electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase. ETF is an heterodimer that consist of an alpha and a beta subunit and which bind one molecule of FAD per dimer. A similar system also exists in some bacteria. The alpha subunit of ETF is a protein of about 32 Kd which is structurally related to the bacterial nitrogen fixation protein fixB which could play a role in a redox process and feed electrons to ferredoxin. Other related proteins are: - Escherichia coli hypothetical protein ydiR. - Escherichia coli hypothetical protein ygcQ. As a signature pattern for these proteins we selected a highly conserved region which is located in the C-terminal section. -Consensus pattern: [LI]-Y-[LIVM]-[AT]-x-[GA]-[IV]-[SD]-G-x-[IV]-Q-[HP]-x(2)- G-x(6)-[IV]-x-A-[IV]-N -Sequences known to belong to this class detected by the pattern: ALL, except for ygcQ. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: December 2004 / Pattern and text revised. [ 1] Finocchiaro G., Ikeda Y., Ito M., Tanaka K. "Biosynthesis, molecular cloning and sequencing of electron transfer flavoprotein." Prog. Clin. Biol. Res. 321:637-652(1990). PubMed=2326318 [ 2] Tsai M.H., Saier M.H. Jr. "Phylogenetic characterization of the ubiquitous electron transfer flavoprotein families ETF-alpha and ETF-beta." Res. Microbiol. 146:397-404(1995). PubMed=8525056 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}