{PDOC00606}
{PS00743; BETA_LACTAMASE_B_1}
{PS00744; BETA_LACTAMASE_B_2}
{BEGIN}
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* Beta-lactamases class B signatures *
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Beta-lactamases (EC 3.5.2.6) [1,2]  are enzymes  which catalyze the hydrolysis
of an amide bond in  the  beta-lactam ring  of  antibiotics  belonging  to the
penicillin/cephalosporin  family.  Four  kinds  of  beta-lactamase  have  been
identified [2].  Class-B enzymes are zinc containing proteins whilst class -A,
C and D enzymes  are  serine hydrolases.  Class-B  beta-lactamases  have  been
described in  several  Gram-negative bacterial species; they seem to share the
characteristic of  being  able to hydrolyze carbapenem compounds which are new
beta-lactam antibiotics of great therapeutic potential.

There are a number of conserved regions in the sequence of known class-B beta-
lactamases [3]. Most of them are centered on residues known [4] to be involved
in binding  the  zinc  ion  essential  for the enzyme's catalytic activity. We
designed two  signature  patterns for this class of enzyme. The first contains
three residues  involved  in  binding zinc ions. The second pattern contains a
cysteine which is also a zinc ligand.

-Consensus pattern: [LI]-x-[STN]-[HN]-x-H-[GSTAD]-D-x(2)-G-[GP]-x(7,8)-[GS]
                    [H/N, H and D are zinc ligands]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Consensus pattern: P-x(3)-[LIVM](2)-x-G-x-C-[LIVMF](2)-K
                    [C is a zinc ligand]
-Sequences known to belong to this class detected by the pattern: ALL,  except
 for L1 from Xanthomonas maltophilia.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Last update: April 2003 / Patterns and text revised.

[ 1] Ambler R.P.
     "The structure of beta-lactamases."
     Philos. Trans. R. Soc. Lond., B, Biol. Sci. 289:321-331(1980).
     PubMed=6109327
[ 2] Bush K.
     "Characterization of beta-lactamases."
     Antimicrob. Agents Chemother. 33:259-263(1989).
     PubMed=2658779
[ 3] Osano E., Arakawa Y., Wacharotayankun R., Ohta M., Horii T., Ito H.,
     Yoshimura F., Kato N.
     "Molecular characterization of an enterobacterial metallo
     beta-lactamase found in a clinical isolate of Serratia marcescens that
     shows imipenem resistance."
     Antimicrob. Agents Chemother. 38:71-78(1994).
     PubMed=8141584
[ 4] Concha N.O., Rasmussen B.A., Bush K., Herzberg O.
     "Crystal structure of the wide-spectrum binuclear zinc beta-lactamase
     from Bacteroides fragilis."
     Structure 4:823-836(1996).
     PubMed=8805566

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