{PDOC00641}
{PS00813; IF4E}
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* Eukaryotic initiation factor 4E signature *
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Eukaryotic translation initiation factor 4E (eIF-4E)  [1]  is  a  protein that
binds to the cap structure of eukaryotic cellular mRNAs. eIF-4E recognizes and
binds the 7-methylguanosine-containing (m7Gppp)  cap  during  an early step in
the initiation of protein synthesis and facilitates ribosome binding to a mRNA
by inducing the unwinding of its secondary structures.

eIF-4E is  a  conserved protein  of  about 25 Kd.  Site  directed  mutagenesis
experiments  have  shown [2]  that  a  tryptophan  in  the central part of the
sequence of human eIF-4E seems to be implicated in cap-binding.  The signature
pattern for eIF-4E includes this tryptophan.

-Consensus pattern: [DE]-[IFYL]-x(2)-F-[KRL]-x(2)-[LIVM]-x-P-x-W-E-[DVA]-x(5)-
                    G-G-[KR]-W
                    [The first W seems to be involved in cap-binding]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: December 2004 / Pattern and text revised.

[ 1] Thach R.E.
     "Cap recap: the involvement of eIF-4F in regulating gene expression."
     Cell 68:177-180(1992).
     PubMed=1733496
[ 2] Ueda H., Iyo H., Doi M., Inoue M., Ishida T., Morioka H., Tanaka T.,
     Nishikawa S., Uesugi S.
     "Combination of Trp and Glu residues for recognition of mRNA cap
     structure. Analysis of m7G base recognition site of human cap binding
     protein (IF-4E) by site-directed mutagenesis."
     FEBS Lett. 280:207-210(1991).
     PubMed=1672854

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