{PDOC00648} {PS00824; EF1BD_1} {PS00825; EF1BD_2} {BEGIN} ********************************************************* * Elongation factor 1 beta/beta'/delta chain signatures * ********************************************************* Eukaryotic elongation factor 1 (EF-1) is responsible for the GTP-dependent binding of aminoacyl-tRNAs to the ribosomes [1]. EF-1 is composed of four subunits: the alpha chain which binds GTP and aminoacyl-tRNAs, the gamma chain that probably plays a role in anchoring the complex to other cellular components and the beta and delta (or beta') chains. The beta and delta chains are highly similar proteins that both stimulate the exchange of GDP bound to the alpha chain for GTP [2]. The beta and delta chains are hydrophilic proteins of around 23 to 31 Kd. Their C-terminal part seems important for the nucleotide exchange activity, while the N-terminal section is probably involved in the interaction with the gamma chain. We have developed two signature patterns for this family of proteins. The first corresponds to an acidic region in the central section; the second, to the C-terminal extremity of these proteins. -Consensus pattern: [DEG](2)-[DEK]-[DE]-[LIVMF]-D-L-F-G -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Consensus pattern: [IV]-Q-S-x-D-[LIVM]-x-A-[FWM]-[DNQS]-K-[LIVM] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Expert(s) to contact by email: Amons R.; wmbamons@rulgl.leidenuniv.nl -Last update: April 2006 / Pattern revised. [ 1] Riis B., Rattan S.I., Clark B.F.C., Merrick W.C. "Eukaryotic protein elongation factors." Trends Biochem. Sci. 15:420-424(1990). PubMed=2278101 [ 2] van Damme H.T.F., Amons R., Karssies R., Timmers C.J., Janssen G.M.C., Moeller W. Biochim. Biophys. Acta 1050:241-247(1990). -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}