{PDOC00657}
{PS00834; OMPTIN_1}
{PS00835; OMPTIN_2}
{BEGIN}
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* Aspartyl proteases, omptin family signatures *
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Escherichia coli  protease  VII  (EC 3.4.21.87) (omptin) (gene ompT) [1] is an
outer-membrane associated   protease  with  a  specificity  for  paired  basic
residues. It  seems  to  be  an  aspartyl protease [2] inhibited by zinc ions.
Omptin is highly similar to the following proteins:

 - Salmonella  typhimurium  outer  membrane   protease E (gene pgtE) [3], also
   specific for paired basic residues.
 - Yersinia pestis coagulase/fibrinolysin  (plasminogen activator) (gene  pla)
   [4]. This  enzyme  is  encoded  on  a  plasmid required  for the pathogenic
   potential of the bacteria. It cleaves and activates plasminogen at the foci
   of infection; this allows plasmin to degrade fibrin barriers thus promoting
   the invasiveness of the plague bacteria.
 - Escherichia coli outer membrane protease ompP [5].

The mature  forms of these enzymes are proteins of about 280 to 300 amino-acid
residues. As signature patterns, we selected two well conserved regions in the
central part of these proteins.

-Consensus pattern: W-T-D-x-S-x-H-P-x-T
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Consensus pattern: A-G-Y-Q-E-[ST]-R-[FYW]-S-[FYW]-[TN]-A-x-G-G-[ST]-Y
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Note: These proteins belong to family A26 in the classification of peptidases
 [6,E1].

-Last update: January 2002 / Text revised.

[ 1] Sugimura K., Nishihara T.
     "Purification, characterization, and primary structure of Escherichia
     coli protease VII with specificity for paired basic residues: identity
     of protease VII and OmpT."
     J. Bacteriol. 170:5625-5632(1988).
     PubMed=3056908
[ 2] Vandeputte-Rutten L., Kramer R.A., Kroon J., Dekker N., Egmond M.R.,
     Gros P.
     EMBO J. 20:5033-5039(2001).
[ 3] Grodberg J., Dunn J.J.
     "Comparison of Escherichia coli K-12 outer membrane protease OmpT and
     Salmonella typhimurium E protein."
     J. Bacteriol. 171:2903-2905(1989).
     PubMed=2651422
[ 4] Sodeinde O.A., Goguen J.D.
     "Nucleotide sequence of the plasminogen activator gene of Yersinia
     pestis: relationship to ompT of Escherichia coli and gene E of
     Salmonella typhimurium."
     Infect. Immun. 57:1517-1523(1989).
     PubMed=2651310
[ 5] Kaufmann A., Stierhof Y.-D., Henning U.
     "New outer membrane-associated protease of Escherichia coli K-12."
     J. Bacteriol. 176:359-367(1994).
     PubMed=8288530
[ 6] Rawlings N.D., Barrett A.J.
     "Families of serine peptidases."
     Methods Enzymol. 244:19-61(1994).
     PubMed=7845208
[E1] https://www.uniprot.org/docs/peptidas

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