{PDOC00708} {PS00911; DHODEHASE_1} {PS00912; DHODEHASE_2} {BEGIN} ******************************************* * Dihydroorotate dehydrogenase signatures * ******************************************* Dihydroorotate dehydrogenase (EC 1.3.3.1) (DHOdehase) catalyzes the fourth step in the de novo biosynthesis of pyrimidine, the conversion of dihydroorotate into orotate. DHOdehase is a ubiquitous FAD flavoprotein. In bacteria (gene pyrD), DHOdease is located on the inner side of the cytosolic membrane. In some yeasts, such as in Saccharomyces cerevisiae (gene URA1), it is a cytosolic protein while in other eukaryotes it is found in the mitochondria [1]. The sequence of DHOdease is rather well conserved and we have developed two signature patterns specific to this enzyme. The first corresponds to a region in the N-terminal section of the enzyme while the second is located in the C- terminal section and seems to be part of the FAD-binding domain. -Consensus pattern: [GSA]-x(4)-[GK]-[GSTA]-[LIVFSTA]-[GST]-x(3)-[NQRK]-x-G- [NHY]-x(2)-P-[RTV] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Consensus pattern: [LIVM](2)-[GSA]-x-G(2)-[IV]-x-[STGDN]-x(3)-[ACV]-x(2)-{A}- {R}-x-{L}-G-A -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: 10. -Last update: April 2006 / Pattern revised. [ 1] Nagy M., Lacroute F., Thomas D. "Divergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts." Proc. Natl. Acad. Sci. U.S.A. 89:8966-8970(1992). PubMed=1409592 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}