{PDOC00756} {PS00983; LY6_UPAR} {BEGIN} ********************************* * Ly-6 / u-PAR domain signature * ********************************* A variety of GPI-linked cell-surface glycoproteins are composed of one or more copies of a conserved domain of about 100 amino-acid residues [1,2]. These proteins are: - Urokinase plasminogen activator surface receptor u-PAR, which binds to u-PA and is responsible for its proteolysis-independent signal transduction activation effects. - The mouse family of Ly-6 T-cell antigens (Ly-6A, -6C, -6E, -6F and -6G). - CD59 (MACIF or MIRL), an inhibitor of the complement membrane attack complex action. - Herpesvirus saimiri CD59-related protein (gene 15). - Mouse thymocyte B cell antigen (ThB). - Squid glycoprotein Sgp-2. U-PAR contains three tandem copies of the domain, while all the others are made up of a single domain. As shown in the following schematic, this conserved domain contains 10 cysteine residues involved in five disulfide bonds - in u-PAR, the first copy of the domain lacks the fourth disulfide bond. +------+ +------------------------+ +---+ | | | | | | xCxxCxxxxxxCxxxxxCxxxxxCxxxxxxxxxxxxxxxxxxCxxxxCxxxxxxxxxxxxxxCCxxxCxxxxxxxx | | | | +---------------------+ +--------------+ 'C': conserved cysteine involved in a disulfide bond. The signature pattern that detects this domain starts towards its extremity and ends at the sixth cysteine. -Consensus pattern: [EQR]-C-[LIVMFYAH]-x-C-x(5,8)-C-x(3,8)-[EDNQSTV]-C-{C}- x(5)-C-x(12,24)-C [The 6 C's are involved in disulfide bonds] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: May 2004 / Text revised. [ 1] Behrendt N., Ploug M., Patthy L., Houen G., Blasi F., Dano K. "The ligand-binding domain of the cell surface receptor for urokinase-type plasminogen activator." J. Biol. Chem. 266:7842-7847(1991). PubMed=1850423 [ 2] Ploug M., Kjalke M., Ronne E., Weidle U., Hoyer-Hansen G., Dano K. "Localization of the disulfide bonds in the NH2-terminal domain of the cellular receptor for human urokinase-type plasminogen activator. A domain structure belonging to a novel superfamily of glycolipid-anchored membrane proteins." J. Biol. Chem. 268:17539-17546(1993). PubMed=8394346 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}