{PDOC00783} {PS01021; COPROGEN_OXIDASE} {BEGIN} ******************************************** * Coproporphyrinogen III oxidase signature * ******************************************** Coproporphyrinogen III oxidase (EC 1.3.3.3) (coproporphyrinogenase) [1,2] catalyzes the oxidative decarboxylation of coproporphyrinogen III into protoporphyrinogen IX, a common step in the pathway for the biosynthesis of porphyrins such as heme, chlorophyll or cobalamin. Coproporphyrinogen III oxidase is an enzyme that requires iron for its activity. A cysteine seems to be important for the catalytic mechanism [3]. Sequences from a variety of eukaryotic and prokaryotic sources show that this enzyme has been evolutionarily conserved. As a signature pattern we selected a highly conserved region in the central part of the sequence; this region contains the only conserved cysteine and is rich in charged amino acids. -Consensus pattern: K-x-[WQA]-[CA]-x(2)-[FYH](2)-x-[LIVM]-x-[HY]-R-x-E-x-R-G- [LIVMT]-G(2)-[LIVM]-F-[FY]-D -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Note: Some bacteria possess an anaerobic form of coproporphyrinogen III oxidase [4] which does not belong to this family. -Last update: April 2006 / Pattern revised. [ 1] Xu K., Elliott T. "An oxygen-dependent coproporphyrinogen oxidase encoded by the hemF gene of Salmonella typhimurium." J. Bacteriol. 175:4990-4999(1993). PubMed=8349542 [ 2] Kohno H., Furukawa T., Yoshinaga T., Tokunaga R., Taketani S. "Coproporphyrinogen oxidase. Purification, molecular cloning, and induction of mRNA during erythroid differentiation." J. Biol. Chem. 268:21359-21363(1993). PubMed=8407975 [ 3] Camadro J.M., Chambon H., Jolles J., Labbe P. "Purification and properties of coproporphyrinogen oxidase from the yeast Saccharomyces cerevisiae." Eur. J. Biochem. 156:579-587(1986). PubMed=3516695 [ 4] Xu K., Elliott T. "Cloning, DNA sequence, and complementation analysis of the Salmonella typhimurium hemN gene encoding a putative oxygen-independent coproporphyrinogen III oxidase." J. Bacteriol. 176:3196-3203(1994). PubMed=8195073 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}