{PDOC00809}
{PS01055; DNA_LIGASE_N1}
{PS01056; DNA_LIGASE_N2}
{BEGIN}
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* NAD-dependent DNA ligase signatures *
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DNA ligase (polydeoxyribonucleotide synthase) is the enzyme that joins two DNA
fragments by catalyzing the formation of an internucleotide ester bond between
phosphate and deoxyribose. It is active during DNA replication, DNA repair and
DNA recombination.  There  are  two  forms  of  DNA  ligase:  one requires ATP
(EC 6.5.1.1), the other NAD (EC 6.5.1.2).

Bacterial DNA ligases are NAD-dependent.  They  are proteins of about 75 to 85
Kd whose sequence is well conserved [1]. They also show similarity to yicF, an
Escherichia  coli  hypothetical protein of 63 Kd.  We  developed two signature
patterns  for this family of proteins; these signatures are based on conserved
regions in the N-terminal half.

-Consensus pattern: K-[LIVMF]-D-G-[LIVMAS]-[SAG]-x(4)-Y-x(2)-[GRD]-x-[LF]-
                    x(4)-[ST]-R-G-[DN]-G-x(2)-G-[DE]-[DENL]
                    [K is the active site residue]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Consensus pattern: [IV]-G-[KR]-[ST]-G-x-[LIVM]-[STNK]-x-[VTLYF]-x(2)-[LVMF]-
                    x-[PS]-[IV]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Last update: December 2004 / Patterns and text revised.

[ 1] Shark K.B., Conway T.
     "Cloning and molecular characterization of the DNA ligase gene (lig)
     from Zymomonas mobilis."
     FEMS Microbiol. Lett. 75:19-26(1992).
     PubMed=1526462
[ 2] Gentry D., Bengra C., Ikehara K., Cashel M.
     "Guanylate kinase of Escherichia coli K-12."
     J. Biol. Chem. 268:14316-14321(1993).
     PubMed=8390989

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