{PDOC00815} {PS01064; PYRIDOX_OXIDASE} {BEGIN} *********************************************** * Pyridoxamine 5'-phosphate oxidase signature * *********************************************** Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5) is a FMN flavoprotein involved in the de novo synthesis of pyridoxine (vitamin B6) and pyridoxal phosphate. It oxidizes pyridoxamine-5-P (PMP) and pyridoxine-5-P (PNP) to pyridoxal-5-P. The sequences of the enzyme from bacterial (genes pdxH or fprA) [1] and fungal (gene PDX3) [2] sources show that this protein has been highly conserved throughout evolution. PdxH is evolutionary related [3] to one of the enzymes in the phenazine biosynthesis protein pathway, phzD (also known as phzG). As a signature pattern, we selected a highly conserved region located in the C-terminal part of these enzymes. -Consensus pattern: [LIVFMYWA]-E-F-W-[QHGENRAMVYLCF]-x(4)-[RW]-[LIVMK]-[HN]- [DNESIT]-R -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: April 2006 / Pattern revised. [ 1] Lam H.-M., Winkler M.E. "Characterization of the complex pdxH-tyrS operon of Escherichia coli K-12 and pleiotropic phenotypes caused by pdxH insertion mutations." J. Bacteriol. 174:6033-6045(1992). PubMed=1356963 [ 2] Loubbardi A., Marcireau C., Karst F., Guilloton M. "Sterol uptake induced by an impairment of pyridoxal phosphate synthesis in Saccharomyces cerevisiae: cloning and sequencing of the PDX3 gene encoding pyridoxine (pyridoxamine) phosphate oxidase." J. Bacteriol. 177:1817-1823(1995). PubMed=7896706 [ 3] Pierson L.S. III, Gaffney T., Lam S., Gong F. "Molecular analysis of genes encoding phenazine biosynthesis in the biological control bacterium. Pseudomonas aureofaciens 30-84." FEMS Microbiol. Lett. 134:299-307(1995). PubMed=8586283 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}