{PDOC00833} {PS01085; RIBUL_P_3_EPIMER_1} {PS01086; RIBUL_P_3_EPIMER_2} {BEGIN} **************************************************** * Ribulose-phosphate 3-epimerase family signatures * **************************************************** Ribulose-phosphate 3-epimerase (EC 5.1.3.1) (also known as pentose-5-phosphate 3-epimerase or PPE) is the enzyme that converts D-ribulose 5-phosphate into D-xylulose 5-phosphate in Calvin's reductive pentose phosphate cycle. In Alcaligenes eutrophus two copies of the gene coding for PPE are known [1], one is chromosomally encoded (cbbEC), the other one is on a plasmid (cbbeP). PPE has been found in a wide range of organisms from all kingdoms of life. The sequence of PPE is highly related to: - Escherichia coli D-allulose-6-phosphate 3-epimerase (gene alsE). - Escherichia coli protein sgcE. All these proteins have from 209 to 241 amino acid residues. As signature patterns, we selected two conserved regions which are located in respectively in the N-terminal and in the central part of these proteins. -Consensus pattern: [LIVMF]-H-[LIVMFY]-D-[LIVM]-x-D-x(1,2)-[FY]-[LIVM]-x-N-x- [STAV] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Consensus pattern: [LIVMA]-x-[LIVM]-M-[ST]-[VS]-x-P-x(3)-[GN]-Q-x(0,1)-[FMK]- x(6)-[NKR]-[LIVMC] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: December 2008 / Text revised. [ 1] Kusian B., Yoo J.G., Bednarski R., Bowien B. "The Calvin cycle enzyme pentose-5-phosphate 3-epimerase is encoded within the cfx operons of the chemoautotroph Alcaligenes eutrophus." J. Bacteriol. 174:7337-7344(1992). PubMed=1429456 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}