{PDOC00856}
{PS01112; RNA_POL_N_8KD}
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* RNA polymerases N / 8 Kd subunits signature *
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In eukaryotes,   there   are   three  different  forms  of  DNA-dependent  RNA
polymerases (EC 2.7.7.6)  transcribing  different sets of genes. Each class of
RNA polymerase  is  an assemblage of ten to twelve different polypeptides.  In
archaebacteria, there  is generally a single form of RNA polymerase which also
consist of an oligomeric assemblage of 10 to 13 polypeptides.

Archaebacterial subunit N (gene rpoN) [1] is a small protein of about 8 Kd, it
is evolutionary related [2] to a 8.3 Kd component shared by all three forms of
eukaryotic RNA polymerases (gene RPB10 in yeast and POLR2J in mammals) as well
as to African swine fever virus protein CP80R [3].

As a signature pattern we selected a conserved  region which is located at the
N-terminal extremity  of  these  polymerase subunits; this region contains two
cysteines that binds a zinc ion [4].

-Consensus pattern: [LIVMFD]-[LIVMFP]-P-[LIVM]-x-C-[FL]-[ST]-C-G
                    [The 2 C's are zinc ligands]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: May 2004 / Text revised.

[ 1] Langer D., Hain J., Thuriaux P., Zillig W.
     "Transcription in archaea: similarity to that in eucarya."
     Proc. Natl. Acad. Sci. U.S.A. 92:5768-5772(1995).
     PubMed=7597027
[ 2] McKune K., Woychik N.A.
     "Halobacterial S9 operon contains two genes encoding proteins
     homologous to subunits shared by eukaryotic RNA polymerases I, II, and
     III."
     J. Bacteriol. 176:4754-4756(1994).
     PubMed=8045907
[ 3] Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
     Rodriguez J.F., Vinuela E.
     "Analysis of the complete nucleotide sequence of African swine fever
     virus."
     Virology 208:249-278(1995).
     PubMed=11831707
[ 4] MacKereth C.D., Arrowsmith C.H., Edwards A.M., McIntosh L.P.
     "Zinc-bundle structure of the essential RNA polymerase subunit RPB10
     from Methanobacterium thermoautotrophicum."
     Proc. Natl. Acad. Sci. U.S.A. 97:6316-6321(2000).
     PubMed=10841539; DOI=97/12/6316

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