{PDOC00866} {PS01125; ROK} {BEGIN} ************************ * ROK family signature * ************************ A family of bacterial proteins has been described [1] which groups transcriptional repressors, sugar kinases and yet uncharacterized open reading frames. This family, known as ROK (Repressor, ORF, Kinase) currently consist of: - Xylose operon repressor (gene xylR) in Bacillus subtilis, Lactobacillus pentosus and Staphylococcus xylosus. - N-acetylglucosamine repressor (gene nagC) from Escherichia coli. - Glucokinase (EC 2.7.1.2) (gene glk) from Streptomyces coelicolor. - Fructokinase (EC 2.7.1.4) (gene scrK or frk) from Pediococcus pentosaceus, Streptococcus mutans and Zymomonas mobilis. - Escherichia coli allokinase (EC 2.7.1.55) (gene alsK). - Escherichia protein mlc. - Hypothetical Escherichia coli protein yajF and HI0182, the corresponding Haemophilus influenzae protein. - Hypothetical Escherichia coli protein yhcI and HI0144, the corresponding Haemophilus influenzae protein. - A hypothetical protein in nagH 5'region from Clostridium perfringens. The repressor proteins (xylR and nagC) from this family possess a N-terminal region not present in the sugar kinases and which contains an helix-turn-helix DNA-binding motif. The domain common to all these proteins consists of about 300 residues. It is not highly conserved, but we could define a pattern centered on a glycine-rich region in the central part of the domain. -Consensus pattern: [LIVM]-x(2)-G-[LIVMFCT]-G-x-[GA]-[LIVMFA]-x(3)-{V}-x(4)-G- x(3,5)-[GATP]-{G}-x-G-[RKH] -Sequences known to belong to this class detected by the pattern: ALL, except for mlc. -Other sequence(s) detected in Swiss-Prot: 5. -Last update: December 2004 / Pattern and text revised. [ 1] Titgemeyer F., Reizer J., Reizer A., Saier M.H. Jr. "Evolutionary relationships between sugar kinases and transcriptional repressors in bacteria." Microbiology 140:2349-2354(1994). PubMed=7952186 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}