{PDOC00877} {PS01140; GLYCOSYL_HYDROL_F45} {BEGIN} ********************************************* * Glycosyl hydrolases family 45 active site * ********************************************* The microbial degradation of cellulose and xylans requires several types of enzymes such as endoglucanases (EC 3.2.1.4), cellobiohydrolases (EC 3.2.1.91) (exoglucanases), or xylanases (EC 3.2.1.8) [1,2]. Fungi and bacteria produces a spectrum of cellulolytic enzymes (cellulases) and xylanases which, on the basis of sequence similarities, can be classified into families. One of these families is known as the cellulase family K or as the glycosyl hydrolases family 45 [3,E1]. The enzymes which are currently known to belong to this family are listed below. - Endoglucanase 5 from Humicola insolens. - Endoglucanase 5 from Trichoderma reesei (egl5). - Endoglucanase K from Fusarium oxysporum. - Endoglucanase B from Pseudomonas fluorescens (celB). - Endoglucanase 1 from Ustilago maydis (egl1). The best conserved regions in these enzymes is located in the N-terminal section. It contains an aspartic acid residue which has been shown [4] to act as a nucleophile in the catalytic mechanism. We use this region as a signature pattern. -Consensus pattern: [STA]-T-R-Y-[FYW]-D-x(5)-[CA] [The D is an active site residue] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Expert(s) to contact by email: Henrissat B.; bernie@afmb.cnrs-mrs.fr -Last update: November 1997 / Pattern and text revised. [ 1] Beguin P. "Molecular biology of cellulose degradation." Annu. Rev. Microbiol. 44:219-248(1990). PubMed=2252383; DOI=10.1146/annurev.mi.44.100190.001251 [ 2] Gilkes N.R., Henrissat B., Kilburn D.G., Miller R.C. Jr., Warren R.A.J. "Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families." Microbiol. Rev. 55:303-315(1991). PubMed=1886523 [ 3] Henrissat B., Bairoch A. "New families in the classification of glycosyl hydrolases based on amino acid sequence similarities." Biochem. J. 293:781-788(1993). PubMed=8352747 [ 4] Davies G.J., Dodson G.G., Hubbard R.E., Tolley S.P., Dauter Z., Wilson K.S., Hjort C., Mikkelsen J.M., Rasmussen G., Schuelein M. "Structure and function of endoglucanase V." Nature 365:362-364(1993). PubMed=8377830; DOI=10.1038/365362a0 [E1] https://www.uniprot.org/docs/glycosid -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}