{PDOC00886} {PS01151; FIMBRIAL_USHER} {BEGIN} ************************************************************** * Fimbrial biogenesis outer membrane usher protein signature * ************************************************************** In Gram-negative bacteria the biogenesis of fimbriae (or pili) requires a two- component assembly and transport system which is composed of a periplasmic chaperone (see ) and an outer membrane protein which has been termed a molecular 'usher' [1,2,3]. The protein is rather large (from 86 to 100 Kd) and seems to be mainly composed of membrane-spanning beta-sheets, a structure reminiscent of porins. Known members of this family are listed below: - Escherichia coli papC for P pili. - Escherichia coli afaC for AFA-III adhesin. - Escherichia coli aggC for AAF/I fimbriae. - Escherichia coli cssD for CS6 antigen. - Escherichia coli faeD for K88 pili. - Escherichia coli fanD for K99 pili. - Escherichia coli fasD for 987P fimbria. - Escherichia coli fimD for type 1 fimbriae. - Escherichia coli focD for F1C fimbriae. - Escherichia coli htrE for a yet unknown pilin. - Escherichia coli CS3-2 for CS3 pili. - Bordetella pertussis fhaA (or fimC) for filamentous hemagglutinin and fimbria. - Haemophilus influenzae hifC for type b pili. - Klebsiella pneumoniae mrkC for type 3 pili. - Proteus mirabilis pmfC for PMF fimbriae. - Salmonella enteritidis sefC for sef14 fimbriae. - Salmonella typhimurium lpfC for long polar fimbriae. - Yersinia enterocolitica myfC for myf fimbriae. - Yersinia pestis caf1A for F1 capsule antigen. - Escherichia coli hypothetical protein ybgQ. - Escherichia coli hypothetical protein ycbS. - Escherichia coli hypothetical protein yehB. - Escherichia coli hypothetical protein yfcU. - Escherichia coli hypothetical protein yhcD. - Escherichia coli hypothetical protein yqiG. - Escherichia coli hypothetical protein yraJ. The degree of sequence similarity of these proteins is not very high. However they share a number of characteristics. One them is the presence of two pairs of cysteines, the first one is located in the N-terminal part and the second at the C-terminal extremity. They are probably involved in disulfide bonds. The best conserved region is located in the central part of these proteins and can be used as a signature pattern. -Consensus pattern: [VL]-[PASQ]-[PAS]-G-[PAD]-[FY]-x-[LI]-[DNQSTAP]-[DNH]- [LIVMFY] -Sequences known to belong to this class detected by the pattern: ALL, except for cssD and sefC. -Other sequence(s) detected in Swiss-Prot: NONE. -Expert(s) to contact by email: Schifferli D.M.; dmschiff@pobox.upenn.edu -Last update: November 1997 / Text revised. [ 1] Jacob-Dubuisson F., Striker R., Hultgren S.J. J. Biol. Chem. 269:12447-12455(1994). [ 2] Schifferli D.M., Alrutz M.A. "Permissive linker insertion sites in the outer membrane protein of 987P fimbriae of Escherichia coli." J. Bacteriol. 176:1099-1110(1994). PubMed=7906265 [ 3] van Rosmalen M., Saier M.H. Jr. Res. Microbiol. 144:507-527(1993). -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}