{PDOC00904} {PS01175; RIBONUCLEASE_II} {BEGIN} ************************************ * Ribonuclease II family signature * ************************************ On the basis of sequence similarities, the following bacterial and eukaryotic proteins seem to form a family: - Escherichia coli and related bacteria ribonuclease II (EC 3.1.13.1) (RNase II) (gene rnb) [1]. RNase II is an exonuclease involved in mRNA decay. It degrades mRNA by hydrolyzing single-stranded polyribonucleotides processively in the 3' to 5' direction. - Bacterial ribnuclease R [2], a 3'-5'exoribonuclease that participates in an essential cell function. - Yeast protein SSD1 (or SRK1) which is implicated in the control of the cell cycle G1 phase. - Yeast protein DIS3 [3], which binds to ran (GSP1) and ehances the the nucleotide-releasing activity of RCC1 on ran. - Fission yeast protein dis3, which is implicated in mitotic control. - Neurospora crassa cyt-4, a mitochondrial protein required for RNA 5' and 3' end processing and splicing. - Yeast protein MSU1, which is involved in mitochondrial biogenesis. - Synechocystis strain PCC 6803 protein zam [4], which control resistance to the carbonic anhydrase inhibitor acetazolamide. - Caenorhabditis elegans hypothetical protein F48E8.6. The size of these proteins range from 644 residues (rnb) to 1250 (SSD1). While their sequence is highly divergent they share a conserved domain in their C- terminal section [5]. It is possible that this domain plays a role in a putative exonuclease function that would be common to all these proteins. We have developed a signature pattern based on the core of this conserved domain. -Consensus pattern: [HI]-[FYE]-[GSTAM]-[LIVM]-x(4,5)-Y-[STALV]-x-[FWVAC]-[TV]- [SA]-P-[LIVMA]-[RQ]-[KR]-[FY]-x-D-x(3)-[HQ] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: December 2001 / Pattern and text revised. [ 1] Zilhao R., Camelo L., Arraiano C.M. "DNA sequencing and expression of the gene rnb encoding Escherichia coli ribonuclease II." Mol. Microbiol. 8:43-51(1993). PubMed=8497196 [ 2] Cheng Z.-F., Zuo Y., Li Z., Rudd K.E., Deutscher M.P. "The vacB gene required for virulence in Shigella flexneri and Escherichia coli encodes the exoribonuclease RNase R." J. Biol. Chem. 273:14077-14080(1998). PubMed=9603904 [ 3] Noguchi E., Hayashi N., Azuma Y., Seki T., Nakamura M., Nakashima N., Yanagida M., He X., Mueller U., Sazer S., Nishimoto T. "Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1." EMBO J. 15:5595-5605(1996). PubMed=8896453 [ 4] Beuf L., Bedu S., Cami B., Joset F. "A protein is involved in accessibility of the inhibitor acetazolamide to the carbonic anhydrase(s) in the cyanobacterium Synechocystis PCC 6803." Plant Mol. Biol. 27:779-788(1995). PubMed=7727754 [ 5] Mian I.S. "Comparative sequence analysis of ribonucleases HII, III, II PH and D." Nucleic Acids Res. 25:3187-3195(1997). PubMed=9241229 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}