{PDOC00906} {PS01177; ANAPHYLATOXIN_1} {PS01178; ANAPHYLATOXIN_2} {BEGIN} ********************************************** * Anaphylatoxin domain signature and profile * ********************************************** Anaphylatoxins [1] are mediators of local inflammatory process that act by inducing smooth muscle contraction. There are three different anaphylatoxins: C3a, C4a and C5a. They are peptides of about 75 amino-acid residues that are derived from the proteolytic degradation of complement C3, C4 and C5 and which contains six disulfide-bonded cysteines [2] (see the schematic representation below). +--------------------+ +-------------|------------+ | | | | | xxxCCxxxxxxxxxxxxCxxxxxxxxxxxxCxxxxxxCCxxx | | +--------------------------------+ 'C': conserved cysteine involved in a disulfide bond. This cysteine-rich region shares similarity with a three times repeated domain found in the mammalian extracellular matrix proteins fibulins 1 and 2 [3,4]. The three disulfide bonds are conserved in the first and last repeats, but the first disulfide bond is missing in the second repeat. Our consensus pattern spans the entire cysteine-rich domain. -Consensus pattern: [CSH]-C-x(2)-[GAP]-x(7,8)-[GASTDEQR]-C-[GASTDEQL]-x(3,9)- [GASTDEQN]-x(2)-[CE]-x(6,7)-C-C [The 6 C's are involved in disulfide bonds] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: May 2004 / Text revised. [ 1] Hugli T.E. "Biochemistry and biology of anaphylatoxins." Complement 3:111-127(1986). PubMed=3542363 [ 2] Huber R., Scholze H., Paques E.P., Deisenhofer J. "Crystal structure analysis and molecular model of human C3a anaphylatoxin." Hoppe-Seyler's Z. Physiol. Chem. 361:1389-1399(1980). PubMed=7439885 [ 3] Argraves W.S., Tran H., Burgess W.H., Dickerson K. "Fibulin is an extracellular matrix and plasma glycoprotein with repeated domain structure." J. Cell Biol. 111:3155-3164(1990). PubMed=2269669 [ 4] Pan T.-C., Sasaki T., Zhang R.-Z., Faessler R., Timpl R., Chu M.L. "Structure and expression of fibulin-2, a novel extracellular matrix protein with multiple EGF-like repeats and consensus motifs for calcium binding." J. Cell Biol. 123:1269-1277(1993). PubMed=8245130 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}