{PDOC00920} {PS01195; PEPT_TRNA_HYDROL_1} {PS01196; PEPT_TRNA_HYDROL_2} {BEGIN} ************************************** * Peptidyl-tRNA hydrolase signatures * ************************************** Peptidyl-tRNA hydrolase (EC 3.1.1.29) (PTH) is a bacterial enzyme that cleaves peptidyl-tRNA or N-acyl-aminoacyl-tRNA to yield free peptides or N-acyl-amino acids and tRNA. The natural substrate for this enzyme may be peptidyl-tRNA which drop off the ribosome during protein synthesis [1,2]. Bacterial PTH has been found [2,3] to be evolutionary related to yeast hypothetical protein YHR189w. PTH and YHR189w are proteins of about 200 amino acid residues. As signature patterns, we selected two conserved regions that each contain an histidine. The first of these regions is located in the N-terminal section, the other in the central part. -Consensus pattern: [FYH]-x(2)-[TN]-[RK]-H-N-x-G-x(2)-[LIVMFAYCT]-[LIVMFA]- [DEN] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Consensus pattern: [GS]-x(3)-H-N-G-[LIVM]-[KR]-[DNS]-[LIVMTC] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: December 2004 / Pattern and text revised. [ 1] Garcia-Villegas M.R., De La Vega F.M., Galindo J.M., Segura M., Buckingham R.H., Guarneros G. EMBO J. 10:3549-3555(1991). [ 2] De La Vega F.M., Galindo J.M., Old I.G., Guarneros G. Gene 169:97-100(1996). [ 3] Ouzounis C., Bork P., Casari G., Sander C. "New protein functions in yeast chromosome VIII." Protein Sci. 4:2424-2428(1995). PubMed=8563640 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}