{PDOC00940}
{PS01223; PROA}
{BEGIN}
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* Gamma-glutamyl phosphate reductase signature *
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Gamma-glutamyl phosphate  reductase  (EC  1.2.1.41) (GPR)  is the  enzyme that
catalyzes the  second  step in the biosynthesis of proline from glutamate, the
NADP-dependent reduction   of  L-glutamate  5-phosphate  into  L-glutamate  5-
semialdehyde and phosphate.

In eubacteria (gene  proA)  and yeast [1] (gene PRO2), GPR is a monofunctional
protein, while  in plants  and mammals, it is a bifunctional enzyme (P5CS) [2]
that consists   of   two  domains:  a  N-terminal  glutamate  5-kinase  domain
(EC 2.7.2.11) (see <PDOC00701>) and a C-terminal GPR domain.

As a  signature  pattern,  we  selected  a  conserved region that contains two
histidine residues. This region is located in the last third of GPR.

-Consensus pattern: [VA]-x(5)-A-[LIVAMTCK]-x-[HWFY]-[IM]-x(2)-[HYWNRFT]-
                    [GSNT]-[STAG]-x(0,1)-H-[ST]-[DE]-x(1,2)-I
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: April 2006 / Pattern revised.

[ 1] Pearson B.M., Hernando Y., Payne J., Wolf S.S., Kalogeropoulos A.,
     Schweizer M.
     "Sequencing of a 35.71 kb DNA segment on the right arm of yeast
     chromosome XV reveals regions of similarity to chromosomes I and
     XIII."
     Yeast 12:1021-1031(1996).
     PubMed=8896266; DOI=10.1002/(SICI)1097-0061(199609)12:10B<1021::AID-YEA981>3.0.CO;2-7
[ 2] Hu C.-A.A., Delauney A.J., Verma D.P.S.
     "A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase)
     catalyzes the first two steps in proline biosynthesis in plants."
     Proc. Natl. Acad. Sci. U.S.A. 89:9354-9358(1992).
     PubMed=1384052

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