{PDOC00945} {PS01230; TRMA_1} {PS01231; TRMA_2} {BEGIN} ************************************************ * RNA methyltransferase trmA family signatures * ************************************************ In Escherichia coli, the trmA protein is a tRNA (uracil-5-)-methyltransferase (EC 2.1.1.35) that catalyzes the S-adenosylmethionine dependent methylation of U54 in all tRNAs. Orthologs of trmA are found in many eubacterial species. A number of uncharacterized homologs of trmA have been found [1]: - Escherichia coli hypothetical protein ygcA and HI0333, the corresponding Haemophilus influenzae protein. - Haemophilus influenzae hypothetical protein HI0958. - Chlamydia trachomatis protein HOM1. - Fission yeast hypothetical protein SpAC4G8.07c. It is probable that ygcA/HI0333 and HI0958 are probably responsible for the methylation of U747 and U1939 in 23S rRNA. In trmA, a cysteine is known to participate in the catalytic mechanism by forming a covalent adduct to C6 of uracil. We selected two conserved regions as signature patterns, the first includes the active site cysteine and the second one, located at the C-terminal end, contains a conserved histidine. -Consensus pattern: [DN]-P-[PAS]-R-x-G-x(14,19)-[LIVMAF]-[LIVMCAFT]-[YAHG]-x- [SAG]-C-[NAMDSYHKGQ]-x(1,2)-[TNKSI] [C is the active site residue] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Consensus pattern: [LIVMF]-[DN]-x-F-P-[QHYWM]-[ST]-x-[HR]-[LIVMFYT]-E -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: April 2006 / Patterns revised. [ 1] Gustafsson C., Reid R., Greene P.J., Santi D.V. "Identification of new RNA modifying enzymes by iterative genome search using known modifying enzymes as probes." Nucleic Acids Res. 24:3756-3762(1996). PubMed=8871555 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}