{PDOC01036} {PS01333; PYRASE_GLU} {PS01334; PYRASE_CYS} {BEGIN} ************************************************** * Pyrrolidone-carboxylate peptidase active sites * ************************************************** Pyrrolidone-carboxylate peptidase (EC 3.4.19.3) (PYRase) (also known as pyroglutamyl peptidase) is the enzyme that selectively removes pyroglutamate (pGlu) from the N-terminus of proteins and peptides. In bacteria and archebacteria PYRase (gene pcp) is a protein of 22-25 kD. It is a cysteine protease with a Cys-His-Glu catalytic triad [1,2]. We developed two signature patterns that respectively include the glutamate and cysteine active site residues. -Consensus pattern: G-x(2)-[GAP]-x(4)-[LIV]-[ST]-x-E-[KR]-[LIVC]-[AG]-x-[NG] [E is the active site residue] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Consensus pattern: [LIVF]-x-[GSAVC]-x-[LIVM]-S-x-[STAD]-A-G-x-[FY]-[LIVN]-C- [DNS] [C is the active site residue] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Note: These proteins belong to family C15 in the classification of peptidases [3,E1]. -Last update: December 2004 / Pattern and text revised. [ 1] Singleton M.R., Isupov M., Littlechild J. "X-ray structure of pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Thermococcus litoralis." Structure 7:237-244(1999). PubMed=10368293 [ 2] Odagaki Y., Hayashi A., Okada K., Hirotsu K., Kabashima T., Ito K., Yoshimoto T., Tsuru D., Sato M., Clardy J. "The crystal structure of pyroglutamyl peptidase I from Bacillus amyloliquefaciens reveals a new structure for a cysteine protease." Structure 7:399-411(1999). PubMed=10196127 [ 3] Rawlings N.D., Barrett A.J. "Families of cysteine peptidases." Methods Enzymol. 244:461-486(1994). PubMed=7845226 [E1] https://www.uniprot.org/docs/peptidas -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}