{PDOC50202}
{PS50202; MSP}
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* Major sperm protein (MSP) domain profile *
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Nematode sperm  are  unusual  amoeboid cells in which motility is not based on
actin, but instead on the major sperm protein (MSP) that polymerizes to form a
cytoskeleton constructed from intermeshed filaments. MSP is a dimeric molecule
that polymerizes  to  form  non-polar  filaments  constructed from two helical
subfilaments that  wind  round  one  another. The filaments then assemble into
larger macromolecular  assemblies  called fibre complexes. MSP is a small (~14
kDa) basic  protein  typically  encoded  by  a  multigene  family  of up to 28
members [1,2,3,4].  An  about  120-amino  acid  domain similar to MSP has been
found in the following proteins:

 - Animal  Vesicle-Associated  Membrane  Protein-associated  (VAMP-associated)
   protein family  of  33  kDa (VAP33). VAP33 is required for neurotransmitter
   release. It  binds  to  the  v-SNARE synaptobrevin/VAMP which is associated
   with vesicle  fusion.  VAP33 has a two-domain structure with its N-terminus
   being highly  homologous  to  MSP,  whereas  its  C-terminus  is based on a
   putative alpha-helical  coiled-coil  combined  with an extremly hydrophobic
   membrane-attachment region [5].
 - Nicotiana  plumbaginifolia  VAP27, a VAP33 homologue. It interacts with the
   resistance protein Cf9 [6].
 - Yeast  inositol  regulator  SCS2,  a  VAP33  homologue.  It is C-terminally
   anchored to the endoplasmic reticulum [7].

The MSP  polypeptide  chain  has an immunoglobulin-like fold based on a seven-
stranded beta  sandwich  measuring approximately 15 A x 20 A x 45 A and having
opposing three-stranded and four-stranded beta sheets (see <PDB:1MSP>) [1].

The profile we developed covers the entire MSP domain.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: October 2002 / First entry.

[ 1] Bullock T.L., Roberts T.M., Stewart M.
     "2.6 A resolution crystal structure of helices of the motile major
     sperm protein (MSP) of Caenorhabditis elegans."
     J. Mol. Biol. 263:284-296(1996).
     PubMed=8913307
[ 2] Baker A.M.E., Roberts T.M., Stewart M.
     "The crystal structure of aminoglycoside-3'-phosphotransferase-IIa, an
     enzyme responsible for antibiotic resistance."
     J. Mol. Biol. 319:491-499(2002).
     PubMed=12051923
[ 3] Haaf A., LeClaire L. III, Roberts G., Kent H.M., Roberts T.M.,
     Stewart M., Neuhaus D.
     "Solution structure of the motile major sperm protein (MSP) of Ascaris
     suum - evidence for two manganese binding sites and the possible role
     of divalent cations in filament formation."
     J. Mol. Biol. 284:1611-1624(1998).
     PubMed=9878374
[ 4] Smith H.E., Ward S.
     "Identification of protein-protein interactions of the major sperm
     protein (MSP) of Caenorhabditis elegans."
     J. Mol. Biol. 279:605-619(1998).
     PubMed=9641981
[ 5] Nishimura Y., Hayashi M., Inada H., Tanaka T.
     "Molecular cloning and characterization of mammalian homologues of
     vesicle-associated membrane protein-associated (VAMP-associated)
     proteins."
     Biochem. Biophys. Res. Commun. 254:21-26(1999).
     PubMed=9920726
[ 6] Laurent F., Labesse G., de Wit P.
     "Molecular cloning and partial characterization of a plant VAP33
     homologue with a major sperm protein domain."
     Biochem. Biophys. Res. Commun. 270:286-292(2000).
     PubMed=10733941; DOI=10.1006/bbrc.2000.2387
[ 7] Kagiwada S., Hosaka K., Murata M., Nikawa J.-I., Takatsuki A.
     "The Saccharomyces cerevisiae SCS2 gene product, a homolog of a
     synaptobrevin-associated protein, is an integral membrane protein of
     the endoplasmic reticulum and is required for inositol metabolism."
     J. Bacteriol. 180:1700-1708(1998).
     PubMed=9537365

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