{PDOC51117} {PS51117; LAMININ_NTER} {BEGIN} ****************************************** * Laminin N-terminal (LN) domain profile * ****************************************** Laminin is a large molecular weight glycoprotein present only in basement membranes in almost every animal tissue. Each laminin is a heterotrimer assembled from alpha, beta and gamma chain subunits, secreted and incorporated into cell-associated extracellular matrices [1]. Basement membrane assembly is a cooperative process in which laminins polymerize through their N-terminal domain (LN or domain VI) and anchor to the cell surface through their G domains (see ). Netrins may also associate with this network through heterotypic LN domain interactions [2]. This leads to cell signaling through integrins and dystroglycan (and possibly other receptors) recruited to the adherent laminin. This LN domain dependent self-assembly is considered to be crucial for the integrity of basement membranes, as highlighted by genetic forms of muscular dystrophy containing the deletion of the LN module from the alpha 2 laminin chain [3]. The laminin N-terminal domain is found in all laminin and netrin subunits except laminin alpha 3A, alpha 4 and gamma 2. The profile we developed covers the whole laminin N-terminal domain. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: April 2005 / First entry. [ 1] Colognato H., Yurchenco P.D. "Form and function: the laminin family of heterotrimers." Dev. Dyn. 218:213-234(2000). PubMed=10842354; DOI=10.1002/(SICI)1097-0177(200006)218:2<213::AID-DVDY1>3.0.CO;2-R [ 2] Yurchenco P.D., Cheng Y.S. "Self-assembly and calcium-binding sites in laminin. A three-arm interaction model." J. Biol. Chem. 268:17286-17299(1993). PubMed=8349613 [ 3] Xu H., Wu X.R., Wewer U.M., Engvall E. "Murine muscular dystrophy caused by a mutation in the laminin alpha 2 (Lama2) gene." Nat. Genet. 8:297-302(1994). PubMed=7874173; DOI=10.1038/ng1194-297 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}