{PDOC51141}
{PS51141; ZF_SBP}
{BEGIN}
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* Zinc finger SBP-type profile *
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SQUAMOSA   promoter   binding   proteins   (SBPs)   form  a  major  family  of
plant-specific transcription factors related to flower development. They share
a  highly  conserved  DNA-binding domain that contains two zinc-binding sites.
Among the 11 possible ligands for the zinc atoms that are conserved in the SBP
zinc  finger, only 8 are used. The SBP zinc finger follows the general pattern
C-x4-C-x16-C-x2-[HC]-x15-C-x2-C-x3-H-x11-C.  Three  other  histidines are well
conserved but not involved in zinc binding [1,2].

In  vitro  experiments  show that the SBP zinc finger preferentially binds the
consensus   sequence   -TNCGTACAA-  [3].  However,  little  is  known  of  the
physiological  functions  of  these putative transcriptional regulators beyond
their ability to bind DNA.

The solution structure of the SBP zinc finger has been solved (see <PDB:1UL4>)
[2].  The  first  four  Cys  or  His coordinate one zinc ion and the last four
coordinate  the  other.  It  can  be viewed as two structural subdomains, each
subdomain  containing  a  single zinc-binding pocket. The N-terminal subdomain
consists  of  two  short  alpha  helices whereas the C-terminal one contains a
three-stranded antiparallel beta-sheet.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: July 2005 / First entry.

[ 1] Klein J., Saedler H., Huijser P.
     "A new family of DNA binding proteins includes putative
     transcriptional regulators of the Antirrhinum majus floral meristem
     identity gene SQUAMOSA."
     Mol. Gen. Genet. 250:7-16(1996).
     PubMed=8569690
[ 2] Yamasaki K., Kigawa T., Inoue M., Tateno M., Yamasaki T., Yabuki T.,
     Aoki M., Seki E., Matsuda T., Nunokawa E., Ishizuka Y., Terada T.,
     Shirouzu M., Osanai T., Tanaka A., Seki M., Shinozaki K.,
     Yokoyama S.
     "A novel zinc-binding motif revealed by solution structures of
     DNA-binding domains of Arabidopsis SBP-family transcription factors."
     J. Mol. Biol. 337:49-63(2004).
     PubMed=15001351; DOI=10.1016/j.jmb.2004.01.015
[ 3] Cardon G., Hohmann S., Klein J., Nettesheim K., Saedler H.,
     Huijser P.
     "Molecular characterisation of the Arabidopsis SBP-box genes."
     Gene 237:91-104(1999).
     PubMed=10524240

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{END}