{PDOC51265}
{PS51265; ZF_DBF4}
{BEGIN}
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* Zinc finger Dbf4-type profile *
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In  eukaryotes,  initiation  of  DNA  replication  requires  the  assembly  of
pre-replication complexes (pre-RCs) on chromatin during the G1 phase. In the S
phase,  pre-RCs  are  activated  by  two protein kinases, Cdk2 and Cdc7, which
results in the loading of replication factors and the unwinding of replication
origins  by  the  MCM  helicase complex [1]. Cdc7 is a serine/threonine kinase
(see <PDOC00100>)that is conserved from yeast to human. It is regulated by its
association with a regulatory subunit, the Dbf4 protein. This complex is often
referred to as DDK (Dbf4-dependent kinase) [2].

DBF4  contains  an  N-terminal  BRCT domain (see <PDOC50172>) and a C-terminal
conserved  region  that  could  potentially  coordinate  one  zinc  atom,  the
DBF4-type  zinc  finger. The zinc finger is important for the interaction with
Cdc7 [3,4].

The profile we developed covers the entire DBF4-type zinc finger.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: November 2006 / First entry.

[ 1] Nasmyth K.
     "Viewpoint: putting the cell cycle in order."
     Science 274:1643-1645(1996).
     PubMed=8984634
[ 2] Duncker B.P., Brown G.W.
     "Cdc7 kinases (DDKs) and checkpoint responses: lessons from two yeasts."
     Mutat. Res. 532:21-27(2003).
     PubMed=14643426
[ 3] Hardy C.F., Pautz A.
     "A novel role for Cdc5p in DNA replication."
     Mol. Cell. Biol. 16:6775-6782(1996).
     PubMed=8943332
[ 4] Dowell S.J., Romanowski P., Diffley J.F.
     "Interaction of Dbf4, the Cdc7 protein kinase regulatory subunit, with
     yeast replication origins in vivo."
     Science 265:1243-1246(1994).
     PubMed=8066465

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