{PDOC51310} {PS51310; VPS28_C} {BEGIN} *********************************** * VPS28 C-terminal domain profile * *********************************** The endosomal sorting complex I required for transport (ESCRT-I) is composed of the three subunits VPS23/TSG101, VPS28 and VPS37. ESCRT-I is recruited to cellular membranes during multivesicular endosome biogenesis and by enveloped viruses such as HIV-1 to mediate budding from the cell. The C-terminal domain of VPS28 is characterized by a high-sequence conservation throughout all kingdoms of life. It does not directly participate in ESCRT-I assembly (see ) but serves as an adaptor module linked to the ESCRT-I complex that connects its function to other components of the VPS machinery, including ESCRT-III VPS20 [1]. The VPS28 C-terminal domain folds independently into a four-helical bundle structure with N- and C-terminal ends juxtaposed to each other (see ). Helices 1, 2 and 4 have the same length, while helix 3 is shorter and packs with an approximate angle of 45 degrees against the remaining three helices. The connecting loop regions between helices 1 and 2 and helices 2 and 3 are short compared with the longer well-ordered loop connecting helices 3 and 4. Because this loop contains a conserved sequence motif, it may be involved in protein-protein interactions [1]. The profile we developed covers the entire VPS28 C-terminal domain. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: May 2007 / First entry. [ 1] Pineda-Molina E., Belrhali H., Piefer A.J., Akula I., Bates P., Weissenhorn W. "The crystal structure of the C-terminal domain of Vps28 reveals a conserved surface required for Vps20 recruitment." Traffic 7:1007-1016(2006). PubMed=16749904; DOI=10.1111/j.1600-0854.2006.00440.x -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}