{PDOC51321}
{PS51321; TFIIS_CENTRAL}
{BEGIN}
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* TFIIS central domain profile *
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Transcription factor  IIS  (TFIIS)  is  a transcription elongation factor that
increases the  overall transcription rate of RNA polymerase II by reactivating
transcription elongation  complexes that have arrested transcription. TFIIS is
a modular  factor  that  comprises  an  N-terminal domain (see <PDOC51319>), a
central domain, and a C-terminal TFIIS-type zinc finger (see <PDOC00383>). The
central domain  is  conserved  in  TFIIS  homologues  and  interacts  with RNA
polymerase II [1,2].

The TFIIS  central  domain comprises a bundle of three helices [1,2] and three
short helices,  which  form  upon Pol II interaction [3] (see <PDB:1PQV>). The
three-helix bundle  is  built  around  a  hydrophobic core composed largely of
three tyrosines  protruding  from  one  face  of the C-terminal helix [1]. The
TFIIS domain  is  separated  from the TFIIS-type zinc finger by an interdomain
linker [1],  which  is  unstructured  in  free TFIIs [2]. Upon Pol II binding,
however, the  linker  forms an alpha-helix, which runs along the Pol II bottom
face [3].

The profile we developed covers the TFIIS central domain and the linker alpha-
helix.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: June 2007 / First entry.

[ 1] Morin P.E., Awrey D.E., Edwards A.M., Arrowsmith C.H.
     "Elongation factor TFIIS contains three structural domains: solution
     structure of domain II."
     Proc. Natl. Acad. Sci. U.S.A. 93:10604-10608(1996).
     PubMed=8855225
[ 2] Olmsted V.K., Awrey D.E., Koth C., Shan X., Morin P.E., Kazanis S.,
     Edwards A.M., Arrowsmith C.H.
     "Yeast transcript elongation factor (TFIIS), structure and function.
     I: NMR structural analysis of the minimal transcriptionally active
     region."
     J. Biol. Chem. 273:22589-22594(1998).
     PubMed=9712887
[ 3] Kettenberger H., Armache K.-J., Cramer P.
     "Architecture of the RNA polymerase II-TFIIS complex and implications
     for mRNA cleavage."
     Cell 114:347-357(2003).
     PubMed=12914699

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