{PDOC51363}
{PS51363; W2}
{BEGIN}
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* W2 domain profile *
*********************

Translation initiation   is   a   sophisticated,  well  regulated  and  highly
coordinated cellular  process  in  eukaryotes, in which at least 11 eukayrotic
initiation factors   (eIFs)   are  included.  The  W2  domain  (two  invariant
tryptophans) is  a region of ~165 amino acids which is found in the C-terminus
of the following eIFs [1-5]:

 - Eukaryotic translation initiation factor 2B epsilon (eIF-2B-epsilon).
 - Eukaryotic translation initiation factor 4 gamma (eIF-4-gamma).
 - Eukaryotic translation initiation factor 5 (eIF-5), a GTPase-activating
   protein (GAP) specific for eIF2.

The W2  domain  has  a globular fold and is exclusively composed out of alpha-
helices (see <PDB:2FUL>) [3-5]. The structure can be divided into a structural
C-terminal core  onto  which the two N-terminal helices are attached. The core
contains two  aromatic/acidic  residue-rich  regions  (AA  boxes),  which  are
important for mediating protein-protein interactions.

The profile we developed covers the entire W2 domain.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: March 2008 / First entry.

[ 1] Koonin E.V.
     "Multidomain organization of eukaryotic guanine nucleotide exchange
     translation initiation factor eIF-2B subunits revealed by analysis of
     conserved sequence motifs."
     Protein Sci. 4:1608-1617(1995).
     PubMed=8520487
[ 2] Aravind L., Koonin E.V.
     "Eukaryote-specific domains in translation initiation factors:
     implications for translation regulation and evolution of the
     translation system."
     Genome Res. 10:1172-1184(2000).
     PubMed=10958635
[ 3] Boesen T., Mohammad S.S., Pavitt G.D., Andersen G.R.
     "Structure of the catalytic fragment of translation initiation factor
     2B and identification of a critically important catalytic residue."
     J. Biol. Chem. 279:10584-10592(2004).
     PubMed=14681227; DOI=10.1074/jbc.M311055200
[ 4] Wei Z., Xue Y., Xu H., Gong W.
     "Crystal structure of the C-terminal domain of S.cerevisiae eIF5."
     J. Mol. Biol. 359:1-9(2006).
     PubMed=16616930; DOI=10.1016/j.jmb.2006.03.037
[ 5] Bieniossek C., Schuetz P., Bumann M., Limacher A., Uson I., Baumann U.
     "The crystal structure of the carboxy-terminal domain of human
     translation initiation factor eIF5."
     J. Mol. Biol. 360:457-465(2006).
     PubMed=16781736; DOI=10.1016/j.jmb.2006.05.021

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