{PDOC51522} {PS51522; ZF_NANOS} {BEGIN} ********************************** * Zinc finger nanos-type profile * ********************************** Nanos is a highly conserved RNA-binding protein in higher eukaryotes and functions as a key regulatory protein in translational control using a 3' untranslated region during the development and maintenance of germ cells. Nanos comprises a non-conserved amino-terminus and highly conserved carboxy- terminal regions. The C-terminal region has two conserved Cys-Cys-His-Cys (CCHC)-type zinc-finger motifs that are indispensable for nanos function [1,2,3]. The structure of the nanos-type zinc finger is composed of two independent zinc-finger (ZF) lobes, the N-terminal ZF1 and the C-terminal ZF2, which are connected by a linker helix (see ) [3]. These lobes create a large cleft. Zinc ions in ZF1 and ZF2 are bound to the CCHC motif by tetrahedral coordination. The profile we developed covers the entire nanos-type zinc finger. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: February 2011 / First entry. [ 1] Mosquera L., Forristall C., Zhou Y., King M.L. "A mRNA localized to the vegetal cortex of Xenopus oocytes encodes a protein with a nanos-like zinc finger domain." Development 117:377-386(1993). PubMed=8223259 [ 2] Subramaniam K., Seydoux G. "nos-1 and nos-2, two genes related to Drosophila nanos, regulate primordial germ cell development and survival in Caenorhabditis elegans." Development 126:4861-4871(1999). PubMed=10518502 [ 3] Hashimoto H., Hara K., Hishiki A., Kawaguchi S., Shichijo N., Nakamura K., Unzai S., Tamaru Y., Shimizu T., Sato M. "Crystal structure of zinc-finger domain of Nanos and its functional implications." EMBO. Rep. 11:848-853(2010). PubMed=20948543; DOI=10.1038/embor.2010.155 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}