{PDOC51535} {PS51535; PESTIVIRUS_NS3PRO} {BEGIN} *************************************************** * Pestivirus NS3 protease (NS3pro) domain profile * *************************************************** The genome of pestiviruses [E1] is a single plus-strand RNA and contains a single large open reading frame (ORF), a 5' untranslated region (5'UTR) and a 3' untranslated region (3'UTR). The ORF encodes a polyprotein, which is processed by viral and cellular proteases into mature proteins (the structural proteins in the N-terminal portion of the polyprotein whilst the replicative (non-structural or NS) proteins constitute the remainder). The pestivirus NS3 protein is a multifunctional protein possessing serine protease, RNA helicase (see ), and a nucleoside triphosphatase (NTPase) activities located in two functionally distinct domains. The N-terminal one-third of pestiviral NS3 primarily serves as a protease to process the viral polyprotein. The helicase and NTPase activities are localized to the C- terminal of NS3 protein. The NS3 protease (NS3pro) domain is of some 180 aa with a catalytic triad of His-Asp-Ser. Positive regulation (activation) is observed by its interaction with NS4A. The pestivirus NS3pro domain forms peptidase family S31 of clan PA [E2]. The profile we developed covers the entire pestivirus NS3pro domain. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: May 2011 / First entry. [ 1] Gorbalenya A.E., Donchenko A.P., Koonin E.V., Blinov V.M. "N-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases." Nucleic Acids Res. 17:3889-3897(1989). PubMed=2543956 [ 2] Ryan M.D., Monaghan S., Flint M. "Virus-encoded proteinases of the Flaviviridae." J. Gen. Virol. 79:947-959(1998). PubMed=9603310 [ 3] Wang P., Wang Y., Zhao Y., Zhu Z., Yu J., Wan L., Chen J., Xiao M. "Classical swine fever virus NS3 enhances RNA-dependent RNA polymerase activity by binding to NS5B." Virus Res. 148:17-23(2010). PubMed=19951725; DOI=10.1016/j.virusres.2009.11.015 [E1] https://viralzone.expasy.org/39?outline=all_by_species [E2] https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=s31 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}