{PDOC51659}
{PS51659; GT23}
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* Glycosyltransferase family 23 (GT23) domain profile *
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The fucosylation  of  glycoconjugates  in  mammalian organisms is related to a
wide variety   of   biological  preocesses,  including  cell  adhesion,  blood
antigens, and  some  severe  diseases  including cancer metastasis, congenital
disorders of  glycosylation,  and  various  microbial  and  virus  infections.
Fucosylation via  alpha1,2-,  alpha1,3-  alpha1,4-, and alpha1,6-linkages, and
protein O-fucosylation  are  accomplished by the cation of specific individual
fucosyltransferases [1].

FUT8, a  eukaryotic  alpha1,6-fucosyltransferase,  catalyzes the transfer of a
fucosyl residue from guanine nucleotide diphosphate (GDP)-beta-L-fucose to the
reducing terminal    N-acetylglucosamine    (GlcNAc)    of   asparagine-linked
oligosaccharides (N-glycan).  The  catalytic  domain  of  FUT8 is structurally
similar to that of NodZ, a bacterial alpha1,6-fucosyltransferase. NodZ plays a
role in  the  synthesis of the Nod factor, which is involved in the nodulation
of legume  roots  for  nitrogen fixing, and is known to catalyze the alpha1,6-
fucosylation of  lipo-chitooligosaccharides  and variations thereof, including
chitooligosaccharides. Both  the  eukaryotic  and bacterial fucoslytransferase
are classified  into  the  GT23 family of Carbohydrate-Active enZYmes [E1] and
share GDP-beta-L-fucose   as   the  donor  substrate.  Although  the  acceptor
substrates are  different,  a  "common"  chitobiose  unit  is contained in the
reducing terminals of both substrates [2].

The GT23 domain is comprised of two structures, a N-terminal open sheet alpha/
beta structure    and  a C-terminal Rossmann fold which is frequently found in
nucleotide binding  proteins  including  glycosyltransferases (see <PDB:2DE0>)
[1,3,4].

The profile we developed covers the entire GT23 domain.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: October 2012 / First entry.

[ 1] Ihara H., Ikeda Y., Toma S., Wang X., Suzuki T., Gu J., Miyoshi E.,
     Tsukihara T., Honke K., Matsumoto A., Nakagawa A., Taniguchi N.
     "Crystal structure of mammalian alpha1,6-fucosyltransferase, FUT8."
     Glycobiology 17:455-466(2007).
     PubMed=17172260; DOI=10.1093/glycob/cwl079
[ 2] Ihara H., Hanashima S., Okada T., Ito R., Yamaguchi Y., Taniguchi N.,
     Ikeda Y.
     "Fucosylation of chitooligosaccharides by human
     alpha1,6-fucosyltransferase requires a nonreducing terminal
     chitotriose unit as a minimal structure."
     Glycobiology 20:1021-1033(2010).
     PubMed=20466647; DOI=10.1093/glycob/cwq064
[ 3] Brzezinski K., Stepkowski T., Panjikar S., Bujacz G., Jaskolski M.
     "High-resolution structure of NodZ fucosyltransferase involved in the
     biosynthesis of the nodulation factor."
     Acta Biochim. Pol. 54:537-549(2007).
     PubMed=17762900
[ 4] Brzezinski K., Dauter Z., Jaskolski M.
     "Structures of NodZ alpha1,6-fucosyltransferase in complex with GDP
     and GDP-fucose."
     Acta Crystallogr. D 68:160-168(2012).
     PubMed=22281745; DOI=10.1107/S0907444911053157
[E1] http://www.cazy.org/GT23.html

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