{PDOC51743}
{PS51743; ALPHAVIRUS_MT}
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* Alphavirus-like methyltransferase (MT) domain profile *
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The alphavirus-like methyltransferase (MT) domain is found in a superfamily of
positive-strand RNA  viruses  that includes alphaviruses, hepatis E virus, and
rubella virus,  that  are  animal  viruses, as well as a large number of plant
viruses: tobra-,  tobamo-,  bromo-,  tymo-, potex-, and carlaviruses and other
groups. The  alphavirus-like  MT  domain is involved in methylation of the cap
during viral  RNA maturation: S-adenosylmethionine (AdoMet) reacts with GTP to
yield  7-methyl-GTP  (m(7)GTP)  and S-adenosylhomocysteine  (AdoHcy).  In  the
presence of AdoMet, GTP also reacts with the alphavirus-like MT domain to form
a unique covalent complex, 7-methyl-GMP-alphavirus-like MT domain [1,2,3].

The alphavirus-like MT domain encompasses four distinct conserved motifs, with
motif I, II and IV containing an invariant His residue, the AspXXArg signature
and an  invariant  Tyr residue, respectively. It is predicted to belong to the
alpha/beta class of domain structure [1,2,3].

Some proteins known to contain a alphavirus-like MT domain are listed below:

 - Hepatitis E virus (HEV) non-structural polyprotein pORF1.
 - Sindbis virus (SINV) non-structural polyprotein.
 - Semliki forest virus (SFV) non-structural polyprotein.
 - Ross river virus (RRV) non-structural polyprotein.
 - O'nyong-nyong virus (ONNV) non-structural polyprotein.
 - Venezuelan equine encephalitis virus (VEEV) non-structural polyprotein.
 - Tobacco rattle virus (TRV) replicase large subunit.
 - Alfalfa mosaic virus (AMV) replication protein 1a.
 - Brome mosaic virus (BMV) replication protein 1a.
 - Cucumber mosaic virus (CMV) replication protein 1a.
 - Beet necrotic yellow vein virus (BNYVV) replicase.
 - Rubella virus (RUBV) non-structural polyprotein p200.
 - Ononis yellow mosaic virus RNA replicase polyprotein.
 - Eggplant mosaic virus RNA replicase polyprotein.
 - Kennedya yellow mosaic virus (KYMV) RNA replicase polyprotein.
 - Turnip yellow mosaic virus RNA replicase polyprotein.
 - White clover mosaic virus (WCMV) RNA replication protein.
 - Narcissus mosaic virus (NMV) RNA replication protein.
 - Potato virus X (PVX) RNA replication protein.
 - Papaya mosaic potexvirus (PMV) RNA replication protein.
 - Foxtail mosaic virus RNA replication protein.
 - Potato virus M (PVM) RNA replication protein.
 - Apple chlorotic leaf spot virus (ACLSV) RNA-directed RNA polymerase.

The profile we developed covers the entire alphavirus-like MT domain.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: January 2015 / First entry.

[ 1] Rozanov M.N., Koonin E.V., Gorbalenya A.E.
     "Conservation of the putative methyltransferase domain: a hallmark of
     the 'Sindbis-like' supergroup of positive-strand RNA viruses."
     J. Gen. Virol. 73:2129-2134(1992).
     PubMed=1645151
[ 2] Ahola T., Laakkonen P., Vihinen H., Kaeaeriaeinen L.
     "Critical residues of Semliki Forest virus RNA capping enzyme involved
     in methyltransferase and guanylyltransferase-like activities."
     J. Virol. 71:392-397(1997).
     PubMed=8985362
[ 3] Vlot A.C., Menard A., Bol J.F.
     "Role of the alfalfa mosaic virus methyltransferase-like domain in
     negative-strand RNA synthesis."
     J. Virol. 76:11321-11328(2002).
     PubMed=12388692

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