{PDOC51774} {PS51774; NAB} {BEGIN} ****************************************************** * Networked (NET) actin-binding (NAB) domain profile * ****************************************************** The Networked (NET) proteins are a superfamily of plant-specific actin-binding proteins which localize simultaneously to the actin cytoskeleton and specific membrane compartments and are suggested to couple these membranes to the actin cytoskeleton in plant cells. The minimal actin binding region, referred to as the NET actin-binding (NAB) domain, represents a new actin binding motif unique to plants with no apparent primary sequence homolgy to previously identified actin binding domains. In Arabidopsis, the NAB domain always starts with three conserved tryptophan residues, WWW, a motif whose worldwide web connection gives added significance to the NET family name. The C terminal half of the domain is very highly conserved, more so than the N terminal [1,2]. The predicted secondary structure of the domain includes three major alpha helices connected by a beta turns with the WWW motif predicted to form a beta sheet [1,2]. The profile we developed covers the entire NAB domain. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: September 2015 / First entry. [ 1] Deeks M.J., Calcutt J.R., Ingle E.K.S., Hawkins T.J., Chapman S., Richardson A.C., Mentlak D.A., Dixon M.R., Cartwright F., Smertenko A.P., Oparka K., Hussey P.J. "A superfamily of actin-binding proteins at the actin-membrane nexus of higher plants." Curr. Biol. 22:1595-1600(2012). PubMed=22840520; DOI=10.1016/j.cub.2012.06.041 [ 2] Hawkins T.J., Deeks M.J., Wang P., Hussey P.J. "The evolution of the actin binding NET superfamily." Front. Plant Sci. 5:254-254(2014). PubMed=24926301; DOI=10.3389/fpls.2014.00254 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}