{PDOC51839} {PS51839; 4FE4S_HC3} {BEGIN} ************************************************** * His(Cys)3-ligated-type [4Fe-4S] domain profile * ************************************************** The following proteins contain a His(Cys)3-ligated-type [4Fe-4S] domain that consists of just two alpha helices separated by a loop region that coordinates a [4Fe-4S] cluster through an unusual H-x(3)-C-x(2)-C-x(5)-C motif that includes one His and three Cys residues (see ) [1,2,3,4,5,6]: - Prokaryotic and mitochondrial NuoG/Nqo3 subunit (75 kDa) of NADH:ubiquinone oxidoreductase (complex I) (see ). - Clostridium pasteurianum of the iron-only hydrogenase (CpI) (EC:1.12.7.2). - Ralstonia eutropha NAD-dependent formate dehydrogenase alpha subunit (FdsA). - Moorella thermoacetica formate dehydrogenase alpha subunit (FdhA). - Desulfovibrio fructosivorans NADP-reducing hydrogenase subunit HndD. - Clostridium pasteurianum - Ralstonia eutropha NAD-reducing [Ni-Fe] hydrogenase (H2:NAD oxidoreductase) (HoxU). Its motif for the [4Fe-4S] cluster is G-x-H-x-C-x(2)-C-x(5)-C. The profile we developed covers the entire His(Cys)3-ligated-type [4Fe-4S] domain. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: July 2017 / First entry. [ 1] Oh J.-I., Bowien B. "Structural analysis of the fds operon encoding the NAD+-linked formate dehydrogenase of Ralstonia eutropha." J. Biol. Chem. 273:26349-26360(1998). PubMed=9756865 [ 2] Peters J.W., Lanzilotta W.N., Lemon B.J., Seefeldt L.C. "X-ray crystal structure of the Fe-only hydrogenase (CpI) from Clostridium pasteurianum to 1.8 angstrom resolution." Science 282:1853-1858(1998). PubMed=9836629 [ 3] Nicolet Y., Lemon B.J., Fontecilla-Camps J.C., Peters J.W. "A novel FeS cluster in Fe-only hydrogenases." Trends Biochem. Sci. 25:138-143(2000). PubMed=10694885 [ 4] Yano T., Sklar J., Nakamaru-Ogiso E., Takahashi Y., Yagi T., Ohnishi T. "Characterization of cluster N5 as a fast-relaxing [4Fe-4S] cluster in the Nqo3 subunit of the proton-translocating NADH-ubiquinone oxidoreductase from Paracoccus denitrificans." J. Biol. Chem. 278:15514-15522(2003). PubMed=12600982; DOI=10.1074/jbc.M212275200 [ 5] Sazanov L.A., Hinchliffe P. "Structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus." Science 311:1430-1436(2006). PubMed=16469879; DOI=10.1126/science.1123809 [ 6] Nakamaru-Ogiso E., Matsuno-Yagi A., Yoshikawa S., Yagi T., Ohnishi T. "Iron-sulfur cluster N5 is coordinated by an HXXXCXXCXXXXXC motif in the NuoG subunit of Escherichia coli NADH:quinone oxidoreductase (complex I)." J. Biol. Chem. 283:25979-25987(2008). PubMed=18603533; DOI=10.1074/jbc.M804015200 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}