{PDOC51875} {PS51877; HAV_P29_PRO} {PS51875; HAV_P48_PRO} {BEGIN} ******************************************************************************************* * Hypovirulence-associated virus (HAV) papain-like proteases p29 and p48 domains profiles * ******************************************************************************************* Hypoviruses are positive-strand RNA mycoviruses that attenuate virulence of their pathogenic fungal hosts [E1]. They employ a gene expression strategy that involves the autocatalytic processing of the N-terminal portion of encoded polyproteins by papain-like protease domains. The prototypic hypovirus CHV-1/EP713, responsible for virulence attenuation (hypovirulence) of the chestnut blight fungus Cryphonectria parasitica, encodes two contiguous open reading frames (ORFs) designated ORF A and ORF B, which contain N-terminal proteases p29 and p48, respectively. Protease p29 functions as a suppressor of the RNA silencing defense response, while p48 is required for viral RNA replication [1,2,3,4]. The HAV papain-like protease p29 is a cysteine protease, which forms the peptidase family C7 [E2]. A Cys and a His catalytic residue are essential for autocatalytic cleavage at a glycine dipeptide clevage site located at the C- terminus of the domain [1,2,4]. The HAV papain-like protease p48 is a cysteine protease, which forms the peptidase family C8 [E3]. A Cys and a His catalytic residue are essential for autocatalytic cleavage between the cleavage site residues Gly and Ala located at the C-terminus of the domain [1,2,4]. The profiles we developed cover the entire HAV papain-like proteases p48 and p29 domains. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: November 2018 / First entry. [ 1] Shapira R., Nuss D.L. "Gene expression by a hypovirulence-associated virus of the chestnut blight fungus involves two papain-like protease activities. Essential residues and cleavage site requirements for p48 autoproteolysis." J. Biol. Chem. 266:19419-19425(1991). PubMed=1918054 [ 2] Choi G.H., Pawlyk D.M., Nuss D.L. "The autocatalytic protease p29 encoded by a hypovirulence-associated virus of the chestnut blight fungus resembles the potyvirus-encoded protease HC-Pro." Virology 183:747-752(1991). PubMed=1853573 [ 3] Deng F., Nuss D.L. "Hypovirus papain-like protease p48 is required for initiation but not for maintenance of virus RNA propagation in the chestnut blight fungus Cryphonectria parasitica." J. Virol. 82:6369-6378(2008). PubMed=18448523; DOI=10.1128/JVI.02638-07 [ 4] Jensen K.S., Nuss D.L. "Mutagenesis of the catalytic and cleavage site residues of the hypovirus papain-like proteases p29 and p48 reveals alternative processing and contributions to optimal viral RNA accumulation." J. Virol. 88:11946-11954(2014). PubMed=25100848; DOI=10.1128/JVI.01489-14 [E1] https://viralzone.expasy.org/594 [E2] https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=C7 [E3] https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=C8 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}