{PDOC51889}
{PS51889; RUBV_NS_PRO}
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* Rubella virus (RUBV) nonstructural (NS) protease domain profile *
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Rubella virus (RUBV) is the etiological agent of a disease known as rubella or
German measles.  RUBV,  a positive sense, single-strand RNA virus, is the sole
member of  the  Rubivirus  genus [E1] in the Togaviridae family [E2] of animal
viruses. The RUBV genome contains two open reading frames (ORFs) encoding five
proteins after postranslational proteolytic processing; two proteins (P150 and
P90) involved  in  viral  replication  are  translated  from the nonstructural
protein ORF  (NSP-ORF)  and  the three structural proteins that form the virus
particle are  translated  from the structural protein ORF (ST-ORF). A cysteine
protease domain  situated  at  the C-terminus of P150 and immediately upstream
from the  cleavage  site within the NS-ORF precursor (P200) is responsible for
self-cleavage of  P200  into P150 and P90. This protease domain, termed the NS
protease domain, is a papain-like protease with catalytic dyad of Cys and His.
The NS protease domain contains an EF-hand Ca(2+) binding motif, which plays a
structural role  in stabilizing the protease at physiological temperatures, as
well as  a structural Zn(2+) binding site coordinated by four cysteines, which
is necessary  for  protease  activity.  The  RUBV  NS  protease  domain  forms
peptidase family C27 [E3] of clan CA [1,2,3,4].

The profile we developed covers the entire RUBV NS protease domain.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: April 2019 / First entry.

[ 1] Chen J.P., Strauss J.H., Strauss E.G., Frey T.K.
     "Characterization of the rubella virus nonstructural protease domain
     and its cleavage site."
     J. Virol. 70:4707-4713(1996).
     PubMed=8676497
[ 2] ten Dam E., Flint M., Ryan M.D.
     "Virus-encoded proteinases of the Togaviridae."
     J. Gen. Virol. 80:1879-1888(1999).
     PubMed=10466783; DOI=10.1099/0022-1317-80-8-1879
[ 3] Liu X., Yang J., Ghazi A.M., Frey T.K.
     "Characterization of the zinc binding activity of the rubella virus
     nonstructural  protease."
     J. Virol. 74:5949-5956(2000).
     PubMed=10846076
[ 4] Zhou Y., Tzeng W.-P., Wong H.-C., Ye Y., Jiang J., Chen Y., Huang Y.,
     Suppiah S., Frey T.K., Yang J.J.
     "Calcium-dependent association of calmodulin with the rubella virus
     nonstructural protease domain."
     J. Biol. Chem. 285:8855-8868(2010).
     PubMed=20086014; DOI=10.1074/jbc.M109.097063
[E1] https://viralzone.expasy.org/626?outline=all_by_species
[E2] https://viralzone.expasy.org/3?outline=all_by_species
[E3] https://www.ebi.ac.uk/merops/cgi-bin/famsum?family=C27

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